虾蟹卵巢“眼柄切除效应”的分子机制探究——以克氏原螯虾为例

Eyestalk ablation can induce ovarian maturation in captive shrimps and crabs (referred to as "eyestalk-ablation effect"). Elucidating the molecular mechanism of this phenomenon has important scientific significance and application prospects. However, presently the molecular mechanism of "eyestalk-ablation effect" still remains understood poorly, although the story of gonad-inhibiting hormone (GIH) was known to people. It is unclear what the reproductive hormones and the signaling pathways in ovary involved in induction of ovarian maturation. Through publications in conjunction with our previous research results from Procambarus clakii as a representative economic crustacean, we proposed an original hypothesis on the molecular mechanism of the ovarian "eyestalk-ablation effect". In this project, we will use a variety of biotechniques, such as 8-plex iTRAQ labeling quantitative proteomics, bioinformatics analysis, RNA interference and western blotting etc., to prove the above assumption. Implementation of this project will enable us to take the lead in getting the research insights on the ovarian "eyestalk-ablation effect" in crustaceans. The results will be very beneficial to promote the development of reproductive physiology and neuroendocrinology in crustacean, and provide theoretical supports and new ideas to the applied research on artificial reproductive regulation of economic shrimps and crabs.?

切除虾蟹类的眼柄组织能诱导卵巢快速发育成熟（简称虾蟹卵巢"眼柄切除效应"），阐明这一现象的分子机制具有重要科学意义和应用前景。然而，目前人们对该分子机制的认识还远不够深入，尚很明确的是与阻断了眼柄性腺抑制激素（GIH）的作用有关，但下游涉及卵巢中哪些重要生殖激素、信号通路等都还不清楚。我们通过文献调研分析，并结合我们前期以克氏原螯虾为代表动物的研究结果，提出了原创性的有关虾蟹卵巢"眼柄切除效应"的分子机制假说。本申请项目中，我们计划继续以克氏原螯虾为研究对象，采用八标iTRAQ标记的定量蛋白质组学结合生物信息学分析、免疫印迹、RNAi等多种生化分子技术，基本验证所提出的科学假设。项目的实施将使我们在国际上率先深入推进虾蟹卵巢"眼柄切除效应"分子调控机理的研究，研究结果将有利于促进虾蟹类生殖生理学及神经内分泌学研究的发展，对于经济虾蟹人工生殖调控方面的应用研究也将提供一定理论支撑和新思路。

切除虾蟹类的眼柄组织能诱导卵巢快速发育成熟（简称虾蟹卵巢“眼柄切除效应”），阐明这一现象的分子机制具有重要科学意义和应用前景。然而，目前人们对该分子机制的认识还不够深入，尚很明确的是与阻断了眼柄性腺抑制激素（GIH）的作用有关，但下游涉及卵巢中哪些重要生殖激素、信号通路等还不清楚。我们通过文献调研分析，并结合前期以克氏原螯虾为代表动物的研究结果，提出了原创性的有关虾蟹卵巢“眼柄切除效应”的分子机制假说。本项目中我们以克氏原螯虾为研究对象，采用了蛋白质组学、免疫印迹、RNAi等多种生化分子生物学技术并结合生物信息学分析，基本揭示出克氏原螯虾卵巢“眼柄切除效应”启动卵巢快速发育的信号传导通路(GnRH signaling pathway → Calcium signaling pathway → Progesterone-mediated oocyte maturation pathway)，明确甲壳动物与脊椎动物可能同样存在促性腺激素释放激素(GnRH)—促性腺激素(FSH、LH)—性类固醇激素(Progesterone、E2)三级内分泌调控机制，基本验证了所提出的科学假设。研究成果推进了虾蟹卵巢“眼柄切除效应”分子机理的研究进展，有利于促进虾蟹生殖生理学及神经内分泌学研究的发展，对于经济虾蟹人工生殖调控方面的应用研究也将提供一定理论支撑和新思路。