基于低温雨雪冰冻模拟环境的运动生理响应及其适应调控物质筛选研究

Abstract: Cryogenic Freezing Rain and Snow weather is a powerful global climate disaster that threatens human beings' survives seriously in recent years. The physiological response(especially Exercise physiological response) of human beings toward disaster of ice and snow is a basic data including medical meteorological forecast and relief protection, which is bottleneck problem that seriously restricts the development of medical meteorological forecast due to its shortage .With the climate warming and life being comfortable， the vulnerability of human beings toward the disaster of ice and snow will become sharper, what's more, the demanding of exogenous adaptation regulation substances will enlarge accordingly. This project determines climatic factors of imitation environment based on the features of climate of cryogenic freezing rain and snow weather disaster. In addition, this paper set the correlation between temperature-humidity index and motion response as breakthrough point, using the research means of sports physiology to observe the exercise physiological response that mice are influenced by artificial imitation environment. Moreover, this project uses molecular biochemistry, in situ end labeling and immunohistochemical technique to make a quantitative orientation analysis about endogenous stress regulation of substance, and then makes a screen about exogenous adaptation regulation substances and provides basis for the choices of the Intervention occasion of cryogenic freezing rain and snow weather disaster. This research could provide the basic data for the exercise physiological response and its adaptive mechanism in the process of cryogenic freezing rain and snow weather. The project also provides the key parameters for the medical meteorological forecast of cryogenic freezing rain and snow weather and the filtering of climate stress agent. Further more, it could offer the references for the physical fitness of the victims who are affected by the climate disasters and the scientific protection of the relief workers.

低温雨雪冰冻是近年严重威胁人类生存的全球性重大气候灾害，人体对冰雪灾害的生理响应（特别是运动生理响应）是医学气象预报和救灾防护亟需的基础数据，这些基础资料的缺乏是严重制约医学气象预报发展的瓶颈问题。人体对冰雪灾害的脆弱性将随气候变暖和生活舒适化进一步凸显，对外源性适应调控物质的需求亦增大。本项目基于低温雨雪冰冻灾害的气候特征确定模拟环境的气候因子，并以温湿度指数与运动响应相关性为切入点，利用运动生理学研究手段观察小鼠对人工模拟环境的运动生理响应，采用分子生物化学、原位末端标记、免疫组化技术对内源性应激调控物质进行定量定位分析，据此筛选外源性适应调控物质，并对低温雨雪冰冻灾害干预时机的选择提供依据。本研究可为低温雨雪冰冻天气过程运动生理响应及其适应机制提供基础数据，为低温雨雪冰冻灾害的医学气候预报和气候应激保护剂筛选提供关键参数，进而为相关气候受灾人员体能预测和救灾人员科学防护提供参考依据。

低温雨雪冰冻灾害属全球性重大气候灾害。近10年来我国南北方几乎每年均遭受低温雨雪冰冻天气的袭扰,2008年南方多省发生的持续性冰冻灾害使我国的气象防灾减灾体系受到极限挑战，冰冻灾害在我国亦属重大气候灾害。低温雨雪冰冻天气过程中生命响应数据是气象防灾减灾体系亟待补充的基础数据，其中运动生理响应数据还是应急救援队伍管理亟需的基础数据,冰冻环境下生命响应数据采集及其规律属全球亟待解决的前沿科学问题。本课题基于天然低温雨雪冰冻灾害的气候特征确定了模拟环境的气候因子，探明了低温冰冻环境下暴露主体的运动生理响应规律。 本研究的拓展和关键技术突破主要体现在：提供了室内模拟研究技术和多时段(暴露全程、暴露间歇期、暴露结束即刻)行为信号采集技术，实现了行为信号和代谢信号同步采集技术的新突破,这些关键技术可为低温冰冻环境下生命响应数据采集及其响应规律拓展研究提供技术支持, 本研究具有确切的远期科学价值。上述重要数据可为低温雨雪冰冻环境下体能评估和行为评价提供基础数据，因而，本研究还具实际应用价值。.气候变暖背景下生物体对低温雨雪冰冻灾害适应有弱化趋势，生活舒适化将进一步凸显人类的脆弱性，人类对外源性干预物质有实际需求。本课题基于低温雨雪冰冻环境下内源性适应性调控物质表达特征，完成了外源性干预物质的组方和功能评价研究,阐明了Ach-E的调控特征，确认了OPG、TGF-β1、IL-16具有多靶点调控特征， 研制出对OPG、TGF-β1、IL-16、Ach-E具有确切干预作用的组合物。本研究还补充研究了暴露主体对外源性物质的需求特征，研制出能增加暴露主体摄氧量的凤眼莲提取物。这些重要研究成果不仅为应急救灾队伍健康管理提供物质储备，还可为满足暴露主体对外源性干预物质的实际需求提供技术储备。克服气候变暖背景下生物体对低温冰冻灾害适应的弱化趋势具有重大科学价值，本研究成果对未来实现这一目标有实质性贡献。