拟南芥ALA4参与高温胁迫应答分子机制的研究

Heat hazard allied with other abiotic stresses is the major factor to induce global crop loss. Recently, the rapidly developing field of heat stress biology focuses on heat stress proteins (Hsfs) and heat shock transcription factors (Hsfs) which are involved in the process of cellular adjustment and responses to heat stress, however,other proteins participating in this physiological process were little reported. Some protein members of plant P-type ATPase superfamily have been verified to be ralated to varied abiotic stress responses, especially, some members of P4-type ATPase protein family has been confirmed to be involved in temperature -tolerance. Therefore, based on the modal plant Arabidopsis, we propose to systematically research on P4-type ATPase protein family member ALA4 which is predicted to be involved in heat stress responses by means of molecular, cell biology and biophysiology; and attempt to explain its function from the perspective of the relationship between lipids translocase/flippase involved in membrane trafficking and the mechanism of heat stress-tolerance in plant, the details include ALA4 localization, physiological and biochemical traits in vivo as well as the function in the process of heat stress responses, and the final research goal is to demonstrate the molecular mechanism of the function of ALA4 in heat stress responses. These results can not only account for the physiological function of ALA4 itself, but also might provide new knowledge, material and method for related research on plant stresses-tolerance pathways, which possess important significance in both theory and practice.

高温胁迫与其它非生物胁迫伤害是造成全球农作物减产的重要因素。目前对高温胁迫应答的研究主要集中在高温刺激后细胞内下游多种热激蛋白和转录因子的调节和响应过程，而对其它功能蛋白参与高温胁迫应答的过程报道较少。植物P型ATP酶超家族中已有多个成员被证实参与多种非生物胁迫应答，特别是P4型ATP酶家族中已有成员被证实与温度耐受相关。因此，本研究拟利用分子生物学、细胞生物学和生理学等方法，以植物模式材料拟南芥为研究材料，尝试通过磷脂转移酶特性和磷脂转移途径参与高温耐受机制的角度去诠释P4型ATP酶家族成员ALA4的特殊功能，并对其定位、体内生理生化特性和参与高温胁迫应答等内容进行系统研究，旨在揭示ALA4参与高温胁迫响应过程的分子机理。本项目研究成果不仅有利于揭示ALA4参与高温胁迫应答的分子机制，并且还有可能为揭示植物逆境耐受途径等相关研究提供新知识、新材料和新方法，具有重要的理论和实践意义。

结题摘要：.全球气候变暖对农作物的影响深远。细胞膜作为植物对温度最初的感受器被广泛研究关注，参与磷脂转运过程的植物P4型ATP酶家族中也已有成员被证实与温度耐受相关。因此，本研究利用分子生物学、细胞生物学和植物生理学等方法，以植物模式材料拟南芥为研究材料，通过研究磷脂转移酶特性和磷脂转移途径参与高温耐受机制的角度诠释P4型ATP酶家族成员ALA4/6的特殊功能，并对其定位、体内生理生化特性和参与高温胁迫应答等内容进行系统研究，发现ALA4/6可以通过影响细胞膜整合性和流动性参与高温胁迫响应过程。本项目研究成果不仅证实ALA4/6参与高温胁迫应答的特殊机制，并且可以为揭示植物逆境耐受途径等相关研究补充新知识、新材料和新方法，具有重要的理论和实践意义。