烟草花叶病毒蛋白-高分子生物杂合嵌段共聚物的构筑及其组装

We propose to synthesize biocomposite block or tri-block copolymers based on tobacco mosaic virus coat proteins (TMVCPs), and study their self-assembly behavior in aqueous solutions. The block copolymer can be prepared either by directly conjugating polymer onto the specific position of TMVCPs or controlled polymerization of monomer on the TMVCPs. The hierarchical polymer/protein assemblies or virus-like structures can be prepared through the self-assembly of these composite block copolymers in aqueous solutions. Specifically, the study for the effect of protein-protein interactions, proteins-polymer interactions, and polymer-polymer interactions on the self-assembled structure will be emphasized. Our study will pave a new way to design and prepare a new kind of protein/polymer assemblies with well defined structure and special function. In addition, the potential application of these assemblies as the carrier for the controlled drug will be investigated.

在烟草花叶病毒蛋白的特定位点通过直接化学接枝高分子或者通过可控聚合方法制备系列病毒蛋白-高分子的两嵌段或三嵌段生物杂合嵌段共聚物，通过在水溶液中的自组装制备新型聚合物复杂胶束以及类病毒组装体。重点研究蛋白-蛋白、蛋白-高分子、高分子-高分子之间的相互作用对组装结构的影响，研究这类杂合嵌段共聚物的自组装过程以及自组装规律，为设计和构建结构确定及功能独特的新型组装体提供新思路，进一步探讨这些组装体在可控药物载体方面的应用。

由于具有明确的分子结构，以蛋白质为构筑基元的组装体是生物医用材料的理想选择。本项目在烟草花叶病毒衣壳蛋白（TMVCP）的特定位点进行化学修饰，通过引入氟尾、弹性蛋白样多肽、细胞穿膜肽，使TMVCP在保持分子结构明确性的前提下获得了自组装驱动力，并分别通过氟氟相互作用、疏水相互作用或静电相互作用自组装为功能性纳米粒子，进一步用于药物递送、基因递送、抗菌等生物医用领域。本项目的完成，为设计和构建结构明确及功能独特的新型组装体提供了新思路和新方法。