伞形科常用辛味中药的成分组合与活血功效关联性研究

One of the bottleneck problems to restrict the combination of Chinese traditional and Western medicine is that it's difficult to organic combinating Traditional Chinese Medicine potency and efficency with modern pharmacological activity and chemical composition.This project profoundly reveals the association of " composition - blood effect " on the basis of preliminary studies. The projiect use chuanxiong rhizome, ligusticum root, Chinese angelica root and pubescent angelica root, cnidium fruit which are Umbrelliferae Michael essien flavour Chinese traditional medicine too groups for the object. And as the indicator of the pharmacological activity with blood circulation , such as relaxing blood vessels, antispasmodic, anti-platelet aggregation.After identification of active ingredients, selecting the main active ingredient, follow the experimental design - efficacy trials - mathematical modeling (model updating) program, to seek the correlation between the chemical composition and a variety of pharmacological activities, analysing the integration mechanism of vaso active by composition , thus profoundly reveals the correlation..With Chinese drugs of Umbelliferae and Chinese drugs with the similar potency as the object, the project reveals the rules of the traditional drug potency associating with the composition and activity, which has important significance not only to the explain the scientific connotation of Chinese drugs, but also to achieving organic combination between traditional cognition and modern research.

中药传统药性、功效与现代药理活性、化学成分之间难以有机结合，是制约中药中西医结合研究的瓶颈问题之一。本项目在前期研究基础上，以伞形科辛味中药川芎、藁本、当归，独活、蛇床子两组为对象，以与活血化瘀相关的药理活性――舒张血管、解痉、抗血小板聚集等为指标，通过辨识有效成分，选取主要活性成分，遵循试验设计-药效试验-数学建模（模型修正）的程序，寻求化学成分组合与多种药理活性之间的关联性，分析成分组合产生血管活性的整合机制，进而深刻揭示"成分组合－活血功效"的关联性。.本项目以伞形科中药且药性基本相同的中药为对象，围绕活血功效，在有限、可控的范围内揭示传统药性与成分、活性的关联规律，不仅对于阐释中药药性的科学内涵，而且对实现传统认识与现代研究之间的有机结合具有重要意义。

中药是药性的载体，化学成分是中药药理活性的物质基础，揭示中药药性－药理效应－化学成分之间的关联规律，对于阐释中药药性的科学内涵，构建基于传统药性的中药质量控制模式和指导中药新药创制，具有重要意义。. 本项目在前期研究基础上，通过建立数学模型，对伞形科辛味中药川芎、藁本、当归的主要活性成分藁本内酯、阿魏酸、丁基苯酞，独活、蛇床子的主要活性成分蛇床子素、欧前胡素、异欧前胡素进行配比优化，并对预测结果进行验证，分别观察两组3种成分组合对离体血管的舒张作用活性，寻求化学成分组合与多种药理活性之间的关联性。在此基础上，探讨成分组合产生血管活性的整合机制，进而深刻揭示“成分组合－活血功效”的关联性。. 研究结果表明，蛇床子素，异欧前胡素，欧前胡素三种成分组合与阿魏酸、丁基苯酞、藁本内酯三种成分组合均对离体血管具有舒张作用，三种成分之间具有协同增效的作用。这种舒张作用靶点在血管内皮和血管平滑肌。血管内皮机制是通过NO-cGMP途径产生，血管平滑肌机制与抑制平滑肌细胞外Ca2+内流相关。.本研究从三个成分、一个模型（离体血管），两个靶点（血管内皮和血管平滑肌）入手，构建了三种成分组合与血管活性的数学模型，确定了最优配比，提出了适合中药配比优化的方法。对深入研究中药药性－药理效应－化学成分之间的关联规律，创新中药质量评价模式、研发中药组分或成分中药具有重要意义。