利用血糖仪构建便携式适配体传感器用于癌症的早期诊断

Cancer is a malignant tumor, which threatens people's lifes at any time. Fortunately, it could be cured by early diagnosis. Hence, early diagnosis of cancer plays an important role in saving patient's life, which has attracted more attention. The present equipment for early diagnosis of cancer is large-scale and exact with professional operation, resulting in high expense and delay cure. In this project, the novel and portable biosensor using the personal glucose meter is planned to design for early diagnosis of cancer. Aptamer, as the molecular recognition elements, will be induced to design different biosensors, and surface morphology analysis (such as atomic force microscope, scanning electron microscoty, ect.) would be used to characterize the properties of the biosensor. Labeled enzymes would catalyze to generate glucose, which could be assayed by the personal glucose meter. In order to improve the sensitivity, cycle amplification of the enzyme and the richment of the nanoparticles would be applied. This novel portable biosensor will be hopeful to use the detection of tumor marker, which can be further applied the early diagnosis of cancer. Further, this proposed model will be provided an idea for the development of equipment (portable, even household) used for the early diagnosis of cancer.

癌症是一种恶性肿瘤，时刻威胁着人类生命，然而对癌症进行及早的诊断往往对挽救病人生命起到了至关重要的作用，因此癌症的早期诊断已经成为全世界关注的问题。目前的检测手段需要大型精密的设备、专业的技术人员以及昂贵的检查费用，导致许多人无法及时得到诊断，从而延误治疗。本项目利用血糖仪，构建了新型的便携式适配体传感器，并将其应用于癌症早期诊断。以适配体为识别元件，构建出不同类型的传感平台，应用多种表面形貌分析手段（如原子力显微镜，扫描电镜等）考查传感器的各项性能；以酶为媒介，催化产生葡萄糖，通过血糖仪进行检测。同时希望利用酶循环技术和纳米颗粒的富集能力来改善检测限。本项目旨在利用这种新型的便携式传感模式用于肿瘤标记物的检测，更进一步应用于癌症的早期诊断，为便携式的，甚至是家用型癌症的早期诊断仪器的开发提供一个新的思维。

目前，对于癌症早期诊断，主要依赖于患者血清中相关肿瘤标记物的定量检测。这一过程需要精密的设备，专业受训的技术人员，昂贵的检查费用及较长的分析时间。为了实现对肿瘤标记物的现场快速检测或床边检测，我们构建了几种便携式的传感器。例如，我们采用便携式的检测设备作为换能器，如商品化血糖仪，手持式pH计及台式电致化学发光检测仪。同时，当下热门的氮化碳纳米材料及无酶化的杂交链扩增反应也应用于传感器的设计。利用设计的便携式生物传感器，我们成功实现对血清中相关肿瘤标记物的快速检测。