补骨脂盐炙缓和燥性炮制原理研究

Processing with salt can bring drugs into Kidney ,inhance the affect to Kidney,and also reduce dryness of TCM . It is the tranditonal theory to reduce dryness with multi-salt.Dryness is one of the important performance about TCM. It has therapeutic effect and also can produce side effects. There is little research to involve about this. The Traditional preprocessing theories about Fructus Psoraleae is the poison because of too dryness . The multi-salt product is to use in clinical. By the supporting of Eleventh Five-years support program, we complete the processing technology and quality standards and pharmacological effects about Fructus Psoraleae. The results show that the Fructus Psoraleae with salt by Sunburn can ease the adverse effects on normal animals and animal models. The scientific hypothesis is "The dryness is related the function about benefit Kidney yang and warm the Spleen to stop diarrhea functions. The dry poison is an extension of the function. make the dry salt Sunburn role of the relaxation and / or with heating." In the theories of TCM, Kdneys is in charge of water ,it is spittle in the fluid.Spleen is in charge of transportation ,it is saliva in the liquid . The research model " pharmacological studies - chemical analysis - specutrum relationship ", use of the spectrum - effect association, the GC-MS or by HPLC-MS, quantitative PCR technology, combined with TCM syndrome, also reflect the efficiency and moistening dryness in pharmacological effect.In genetic level, multi-level, multi-angle clarify the material basis and mechanism of action to ease dryness of the Fructus Psoraleae with salt by Sunburn. Provide scientific application for clinical to use Fructus Psoraleae reasonable. Through this subject, it is useful to understand the preprocessing theories and also to promote the development of TCM.

盐炙能引药入肾，增强补肾作用，也能降低药物燥性。盐炙润燥是传统炮制理论。燥性是中药性能之一，既起治疗作用也能产生副作用。目前研究资料较少涉及炮制对燥性影响。传统理论认为补骨脂"性本大燥毒"，临床多用盐炙品。课题组在国家"十一五支撑计划"资助下，完成了补骨脂炮制工艺和质量标准以及药理作用研究，结果表明补骨脂盐炙能缓和对正常动物和模型动物的不良影响。提出"燥性与补骨脂补肾助阳，温脾止泻功能有关，燥毒是功能的延伸，盐炙能使燥性作用缓和和/或与加热、加盐有关系"科学假说，基于中医理论"肾主水，在液为唾"和"脾主运化，在液为涎"，拟采用"药理研究-化学分析-谱效关系"研究模式，并采用谱-效关联、荧光定量PCR技术等，结合中医证，并在药理效应中体现增效与润燥，深入基因水平，多层次、多角度阐明补骨脂盐炙缓和燥性的炮制原理，为补骨脂临床合理应用提供科学依据。通过本课题，有利于全面深入认识炮制理论，促进中药炮制学科发展。

盐炙是中药常用的炮制方法，具有引药入肾，增强补肾作用，缓和药物燥性。本课题以补骨脂为研究对象，通过研究阐明盐炙缓和燥性原理。结合临床用药实际情况，采用肾阳虚和脾虚大鼠进行研究，以脏器指数和生化指标进行评价，研究了补骨脂盐炙作用以及与盐炙工艺、剂量关系；产生燥毒主要部位；主要特征成分与燥毒的相关性；并从基因水平探讨了盐炙润燥原理。研究结果表明：补骨脂具有燥毒之性，盐炙能增效、缓和燥毒之性，燥毒之性与剂量有关；补骨脂炮制作用与盐炙工艺加热、加盐相关；燥毒之性的物质存在于多个提取部位，主要为乙酸乙酯部位，是多成分综合作用；通过调整机体与伤阴相关的物质以及在基因水平调整多部位水通道蛋白mRNA表达量达到盐炙缓和燥性作用。通过本课题研究，验证了补骨脂传统理论 “性本大燥毒”的合理性，盐炙能缓和燥性，为临床合理选用炮制品提供依据；阐明补骨脂盐炙缓和燥毒之性的科学内涵，并为研究炮制对药性的影响提供思路。