NONHSAT173448.1通过调控TLR7影响SLE DCs表型及功能的机制研究

T lymphocyte subsets imbalance plays a crucial role in the pathogenesis of systemic lupus erythematosus (SLE), which is mainly mediated by the body's strongest antigen-presenting cells--dendritic cells (DCs), but the specific mechanism remains to be further explored. Recently, long non-coding RNAs (lncRNA) have been demonstrated to play an important role in the immune system as a new "dimension" of regulating life; however, it is unclear whether lncRNA can participate in SLE pathogenesis by regulating the phenotype and function of DCs. Our previous study found that the specifically low expression of lncRNA NONHSAT173448.1 in SLE DCs is closely related to SLE disease activity, in addition, it could regulate the expression level of its intracellular receptor Toll like receptor (TLR7) and affect the immunoregulatory function of DCs on follicular helper T cells (Tfh) and regulatory T cells (Treg). This project intends to investigate the effect of NONHSAT173448.1 on the phenotype and function of DCs in SLE patients, to explore its possible mechanism, to provide clues for the pathogenesis and new treatment options for SLE patients from the perspective of epigenetics and molecular biology.

T淋巴细胞亚群失衡在系统性红斑狼疮（SLE）发病中至关重要，这一过程主要由功能强大的抗原递呈细胞—树突状细胞（DCs）介导，但具体机制尚待深入研究。近期研究发现，长链非编码RNA（lncRNA）作为调控生命的全新“维度”，在免疫系统中发挥重要作用，但其能否通过调控DCs表型及功能参与SLE发病尚未可知。我们前期研究表明，特异性低表达于SLE患者外周血DCs中的lncRNA NONHSAT173448.1与SLE患者疾病活动度密切相关，可影响其胞内Toll样受体（TLR7）表达水平，并可调控DCs免疫调节滤泡辅助T细胞（Tfh）、调节性T细胞（Treg）的功能。本项目拟深入研究NONHSAT173448.1能否通过调控TLR7影响SLE患者DCs表型及功能参与其发病，探索可能的作用机制，为SLE患者发病机制及治疗提供新的线索。

T淋巴细胞亚群失衡在系统性红斑狼疮（SLE）发病中至关重要，这一过程主要由功能强大的抗原递呈细胞—树突状细胞（DCs）介导，但具体机制尚待深入研究。近期研究发现，长链非编码RNA（lncRNA）作为调控生命的全新“维度”，在免疫系统中发挥重要作用，但其能否通过调控DCs表型及功能参与SLE发病尚未可知。我们研究表明，特异性低表达于SLE患者外周血DCs中的lncRNA NONHSAT173448.1与SLE患者疾病活动度密切相关，可影响其胞内Toll样受体（TLR7）表达水平，并可调控DCs免疫调节滤泡辅助T细胞（Tfh）及调节性T细胞（Treg）。本项目深入研究NONHSAT173448.1能否通过调控TLR7影响SLE患者DCs表型及功能参与其发病，为SLE患者发病机制及治疗提供新的线索。