基于微流控技术的生化需氧量电化学检测芯片研究

Aiming at the problems of long response time and complicated procedure for biochemical oxygen demand detection in water, a research of electrochemical detection chip for biochemical oxygen demand based on microfluidic technology is proposed. To solve the problems of long response time and low sensitivity, high efficient immobilization mechanism and method of microorganisms in microchannel are studied, leading to the construction of sensitive interface and high biochemical signal transfer efficiency. The dissolved oxygen detection mechanism and method based on ultra micro electrode and nanomaterial are studied to obtain rapid and sensitive response. To solve the problem of complicated detection procedure, the microfluidic chip’s structure design and fabrication technology are studied to set up a microfluidic chip detection platform. The integration method of microfluidic chip and biochemical oxygen demand detection technology is studied to obtain a high performance biochemical oxygen demand electrochemical detection chip. The development of the proposed detection chip will provide theoretical support and technical method for biochemical oxygen demand’s rapid, sensitive, integrated and automated detection.

针对水中生化需氧量检测时间长、检测过程繁琐的问题，本项目提出了一种基于微流控技术的生化需氧量电化学检测芯片的研究。针对生化需氧量检测时间长、灵敏度低的问题，通过研究微生物于微流道内的高效固定机理和方法，构建传感敏感表面，提高生化信号转换效率；通过研究基于超微电极及复合纳米材料的溶解氧检测机理与方法，获得快速灵敏的响应信号。针对生化需氧量检测过程繁琐的问题，通过研究微流控芯片的结构设计和制造技术，搭建最佳的微流控芯片检测平台；通过研究微流控芯片与生化需氧量检测技术的集成方法，获得一种高性能的生化需氧量电化学检测芯片。通过本项目的实施，为生化需氧量的快速、灵敏、集成化以及自动化检测提供有效的理论支持和技术手段。

针对地表水中生化需氧量检测时间长、检测过程繁琐的问题，本项目提出了一种基于微流控技术的生化需氧量电化学检测芯片研究。研究了具有三维结构内表面的微流道的结构设计及制备方法，研究了超微电极阵列的扩散特性以及片上三电极体系的构建方法，开展了超微电极表面复合纳米材料的修饰方法及敏感机理研究，结合微流道和三电极检测体系，研制生化需氧量检测芯片，完成了生化需氧量的快速检测。该研究为生化需氧量的快速、灵敏、集成化以及自动化检测提供了有效的理论支撑和技术手段。