CYP6CX1、CYP4C1基因在烟粉虱对噻虫嗪抗性中的功能特性研究

Research on the thiamethoxam resistance mechanism to Bemisia tabaci will be provide foundation for pest control in the field，novel pesticides innovation and carry out the high quality resistance genes. Our previous efforts showed that PNOD activities of the thiamethoxam resistance strains were higher than that of susceptible strains. We also carried out two P450 genes, Cyp6cx1 and Cyp4c1 genes were highly overexpressed in thiamethoxam resistance strains. The propose of this research was based on the previous study and preliminary experimental data, we inferred that Cyp6cx1 and Cyp4c1 genes overexpressed in B. tabaci could be accelerated the metabolic ability to thiamethoxam, then affected the resistance generated. In this research, gene interaction, RNA interference (RNAi), RT-PCR and UPLC-MS/MS analysis were carried out our hypothesis. The findings generated from this study will reveal the resistance mechanism in whiteflies. In addition, this study provided a solid foundation to explore resistance genes and promote the development and sustainable application of novel pesticides.

烟粉虱对噻虫嗪抗性分子机制的研究对阐明害虫的抗性机制、新农药的创制及发掘优质的抗性基因具有重要意义。前期我们通过对B烟粉虱噻虫嗪抗性和敏感品系研究发现，细胞色素P450多功能氧化酶活性在抗性品系中要显著高于敏感品系；进而从基因和蛋白两个层面在烟粉虱抗、敏品系间筛选到2个差异表达基因—Cyp6cx1和Cyp4c1。本项目的提出是基于以上初步研究结果和预实验数据，我们推测Cyp6cx1和Cyp4c1这两个基因的过量表达可能加速了烟粉虱对噻虫嗪的代谢能力，从而进一步影响了抗性的产生。我们拟通过功能互补、基因敲除、RT-PCR、UPLC-MS等多种求证技术验证我们的假设。这一问题的解决将明确烟粉虱对噻虫嗪的抗性分子机制，为挖掘优质的抗性基因及有效利用和开发新农药提供重要的理论和实践依据。

烟粉虱对噻虫嗪抗性分子机制的研究对阐明害虫的抗性机制、新农药的创制及发掘优.质的抗性基因具有重要意义。前期我们通过对B烟粉虱噻虫嗪抗性和敏感品系研究发现，.细胞色素P450多功能氧化酶活性在抗性品系中要显著高于敏感品系；进而从基因和蛋白两.个层面在烟粉虱抗、敏品系间筛选到2个差异表达基因—Cyp6cx1和Cyp4c1。本项目的提出.是基于以上初步研究结果和预实验数据，我们推测Cyp6cx1和Cyp4c1这两个基因的过量表.达可能加速了烟粉虱对噻虫嗪的代谢能力，从而进一步影响了抗性的产生。我们拟通过功.能互补、基因敲除、RT-PCR、UPLC-MS等多种求证技术验证我们的假设。这一问题的解决.将明确烟粉虱对噻虫嗪的抗性分子机制，为挖掘优质的抗性基因及有效利用和开发新农药.提供重要的理论和实践依据。