鄱阳湖水系磺胺类抗生素自表达特性分子印迹光子晶体阵列传感器构建及响应机理

Sulfonamides, the synthetic antibiotics, are the most widely used as veterinary antibiotics in animal husbandry and aquaculture. Sulfonamides were excreted from the environment in parent or metabolite via animal manure, leading to a selective pressure on the environmental bacteria and inducing the formation of antibiotic resistant bacteria，which will threaten the human health potentially by food chains. Therefore, there have great significanec to develop a noval analytical protocols based on efficient analytical processes (sensitive, selective, fast and inexpensive).In this project, combination of interaction response characteristics of molecularly imprinting and the signal self-reporting features of photonic crystals the molecularly imprinted photonic film was rationally designed assisted by molecular dynamics sitmulations and chemometrics. Finally, a signal self-reporting sensor array was fabricated directly based on the cross-reactive molecularly imprinted photonic film to detect sulfonamides in Jiangxi Poyang Lake water. The fabricated sulfonamides sensor array can simultaneously possess a serials of desirable features, including high sensitivity and specificity, quick response, well regenerative ability and signal self-reporting characteristics, the color difference can be easily observed with the naked eye. Therefore, this method provides a theoretical guidance for rational design of a signal self-reporting photonic molecular imprinting system. This approach will be a potential way for detecting sulfonamides at trace levels and it could be used as sinificant indicators for pollution level at Jiangxi Poyang Lake. The research will have both scientific and practiacl sinificances for Jiangxi economic development with efficent and green way.

磺胺类抗生素是畜牧业和水产养殖业中广泛使用的抗菌药物，它们在环境中残留并长期存在，将胁迫微生物产生耐药性，进一步通过食物链传递到人体，威胁人类生命安全。因此，发展一种创新的灵敏、选择性强、快速、经济的分析方法具有重要意义。本项目拟将分子印迹技术和光子晶体相结合，利用分子印迹聚合物交互响应的特性和光子晶体信号自表达的特性，用分子动力学模拟、化学计量学和响应曲面法合理设计和优化分子印迹光子晶体薄膜性能，构建具有磺胺类抗生素自表达特性分子印迹光子晶体阵列传感器，实现对江西鄱阳湖水中磺胺类抗生素的信号自表达检测。并对其传感性能进行测试、表征，所构建的传感器阵列具有高灵敏度、高选择性、快速响应、可再生性以及信号自表达等传感特性，可方便用裸眼观察到颜色变化，为快速痕量检测磺胺类抗生素供技术支撑。成果将直接服务于江西鄱阳湖区域地方经济，具有重要的科学意义和实际应用价值。

本项目将分子印迹技术和光子晶体相结合，利用分子印迹聚合物交互响应的特性和光子晶体信号自表达的特性，构建具有磺胺类抗生素自表达特性分子印迹光子晶体阵列传感器。运用分子动力学模拟、化学计量学和响应曲面法等手段筛选合适的模板分子、功能单体、交联剂和致孔剂以及最佳配比；并进一步和合适粒径的SiO2微球构建自表达特性分子印迹光子晶体膜；对晶体膜进行SEM、传感性能、选择性、响应时间、可重复性、吸附动力学和结合常数等表征后优化分子印迹光子晶体薄膜性能并构建具有高灵敏度、高选择性、快速响应、可再生性以及信号自表达等传感特性的传感器阵列，实现对江西鄱阳湖水中磺胺类抗生素的信号自表达检测。在本项目顺利进行的同时，我们还构建了分子印迹比率荧光纳米传感器用于叶酸的快速可视化检测；基于二氧化锰纳米片的比率荧光和比色法双模式用于可视化检测碱性磷酸酶活性；具有选择性识别和提取雌二醇的可切换拉链式热响应分子印迹聚合物等。