脐血干细胞归巢的相关研究

The influence of various transplantation methods on the homing of umbilical cord stem cells were systemically observed, including processing acceptor or not, transplantation routes and programmed transplantation. Based on the results, the effects of the hemotoplastic microenvironmental ingredients on the homing were explored through transplantating umbilical cord CD34+ cells with fetal hemotoplastic matrix stem cells simultaneouslly, or after incubation with cytokines (IL-3, GM-CSF, SCF, FGF) and cellular matrix components (Fn, HSPG) three hours in vitro.

分离并标记人脐血CD34+细胞后，采用程序性移植方法、从不途径（静脉、腹腔、门静脉）浦踩隨CID小鼠内，分别改善造血微环境（输注胎肝基质细胞；与IL-3、GM-SCF、SCF、FGFn、HSPG孵育），检测CD34+在骨髓、CD3-CD4-CD8-细胞在胸腺中的归巢情况。探讨促进造血和免疫重建的干细胞移植新途径。探讨促进造血免和疫重建的干细胞移植新途径、新方法、对细胞分化和细胞免疫的研究具有一定