典型钨矿区土壤多聚钨酸盐类污染物分布特征及其对水稻致毒的分子机理

As a class of newly recognized evironmental pollutants,tungsten and its compounds have been drawing global attention regarding their ecological safety. However, the study of tungsten toxicology is still in its infancy, and the knowledge so far about the species, toxicity and toxicology of environmental tungsten compounds, is extremely limited. Although China has long been known for its abundance in resource and overwhelming exploitaion of tungsten mines, systematic study concerning the ecological and evironmental safety of tungsten in China is yet to be initiated. Tungsten mine-concentrated areas in China are naturally the most significant sources and probably also the severest sufferer of tungsten-related pollution, and in the meantime, the soils in these areas likely contain the most diverse forms of pollutive tungsten compound species. Therefore, in this programm we propose to use soils collected from a representative tungsten mine-concentrated area, as a model system, for study of tungsten compounds in the environment, while the research emphasis is focussed on PTS (polytungstates), a class of tungsten compouds demonstrating potentially the highest level of ecological toxicity. The objective of the present study include :characterization of (1) the species and distribution of PTS in soils of a representative tungsten mine-concentrated area, and of (2) the toxicity of PTS to rice-the main local crop, and (3) on these ground,explore the molecular mechanism of PTS toxication to crops using the strategy of differential proteomics. The expected research results could provide valuable references for the establishment of sound regulations, prevention and control measures for tungsten-related pollution.

钨及其化合物作为一类新兴的环境污染物，其生态安全性已引起全球广泛关注。由于研究历史较短，人们对生态环境中可能存在的含钨化合物的种类、生态毒性和致毒机理认识不深。我国作为世界钨资源中心和开发利用大国，对钨的生态安全性方面的研究却还处于起步阶段。钨矿矿集区是我国钨污染的主要来源，其土壤中种类丰富的含钨化合物则必然是我国钨污染的主要形式。因此，本申请提出以典型钨矿矿集区土壤为切入点，作为研究我国生态环境中钨污染的模式体系，着重探明其中潜在生态毒性最大的多聚钨酸盐(PTS)类污染物的组成分布，及其与源土壤背景属性之间的相关性和衍生规律；并以矿区代表性农作物水稻为供试材料分析各获表征的PTS的生态毒性；在此基础上，采用比较蛋白质组学研究策略，分离、表征和鉴定作物（水稻）中对PTS胁迫响应的差异表达蛋白，结合生物信息学分析，揭示PTS对农作物（水稻）致毒的分子机理。

钨及其化合物作为一类新兴的环境污染物，其生态安全性已引起全球广泛关注。由于研究历史较短，人们对生态环境中可能存在的含钨化合物的种类、生态毒性和致毒机理认识不深。我国作为世界钨资源中心和开发利用大国，对钨的生态安全性方面的研究却还处于起步阶段。钨矿矿集区是我国钨污染的主要来源，其土壤中种类丰富的含钨化合物则必然是我国钨污染的主要形式。因此，本项目以典型钨矿矿集区土壤为切入点，作为研究我国生态环境中钨污染的模式体系，着重探明其中潜在生态毒性最大的多聚钨酸盐(PTS)类污染物的组成分布，及其与源土壤背景属性之间的相关性和衍生规律；并以矿区代表性农作物水稻为供试材料分析各获表征的PTS的生态毒性；在此基础上，采用比较蛋白质组学研究策略，分离、表征和鉴定作物（水稻）中对PTS胁迫响应的差异表达蛋白，结合生物信息学分析，揭示PTS对农作物（水稻）致毒的分子机理。