微波调控骨缺损修复低氧环境的敏感材料设计与机制研究

In clinical and scientific research of bone repair, the hypoxia environment in the bone defect area caused by the changes of bone physiology structure and vascular injury is an important factor to restrict cell activity and osteogenic differentiation. How to effectively improve the hypoxic environment is a key scientific problem in this field. This project design a kind of microwave response compound functional material according to the principle of material prepared on demand. Using tricalcium phosphate bioceramic as the core material, compounding with copper oxide, using the microwave technology to regulate the release of oxygen and copper ions, to improve the hypoxic environment in bone defect area and to promote osteogenic differentiation and improve the bone repair ability. The composite is obtained by mixing tricalcium phosphate powder and the precursor of copper. The physical and chemical properties and biological safety of the composite will be evaluated. Under microwave irradiation, the composite can release oxygen and promote osteogenic differentiation of adipose mesenchymal stem cells. The animal model of bone defect will be constructed to evaluate the bone repair ability of the composite under microwave irradiated through the methods of radiological technology and immunohistochemical staining. This project propose the strategy of using microwave technology to improve the hypoxic environment firstly, according to the character that the sensitive-materials can release oxygen under microwave irradiation. It is a practice to open up a new technique of bone repair materials and promote the development of composite bone repair materials.

在骨修复领域的临床和科研工作中，骨缺损区域内由骨生理结构改变和血管损伤等因素造成的低氧环境，是限制细胞活性和成骨分化的重要因素。如何有效改善低氧环境是该领域亟待解决的关键科学问题。本项目遵循材料按需制备的原则，设计一种微波响应复合功能材料。该材料以磷酸三钙生物陶瓷为核心，通过与氧化铜复合，利用微波技术调控氧化铜释放氧气和铜离子，改善骨缺损区域的低氧环境，促进促进成骨分化，提高骨修复能力。通过磷酸三钙粉体与铜的前驱体混合吸附得到复合材料；评价表征复合材料的理化性能和生物安全性；微波辐照下实现氧化铜磷酸钙复合材料控释氧气，促进脂肪间充质干细胞的成骨分化；构建骨缺损动物模型，利用放射医学和免疫组化染色技术，评价微波辐照氧化铜磷酸钙复合材料的骨修复能力。本项目以敏感响应材料在微波辐照下产氧这一特性，首次提出利用微波技术改善骨缺损修复低氧环境的策略，是骨修复材料应用技术的一次全新实践。

在骨修复领域的临床和科研工作中，骨缺损区域内由骨生理结构改变和血管损伤等因素造成的低氧环境，是限制细胞活性和成骨分化的重要因素。本项目遵循材料按需制备的原则，在氧化铜复合材料成功制备并表征成功的基础上，制备了氧化铜磷酸钙复合材料。从细胞和小鼠两个方面对该复合材料进行了毒性表征。从体外、体内对材料提高富氧的能力进行了研究。发现在微波照射下，制备的复合纳米材料产生的氧气可以显着改善细胞的再氧合程度，可以缓解细胞的乏氧状态，改善骨缺损区域的低氧环境，促进促进成骨分化，提高骨修复能力。