拟南芥CLE信号途径新因子AP2C1基因的分离鉴定及功能分析

Most of their plants organs form through post-embryogenetic development. As the source of generated new organs, stem cells are constantly maintained at various types of meristems throughout the life of a plant. The CLAVATA3(CLV3)/ESR-related (CLE) peptide signals play a key role in the regulation of stem cell homeostasis, organogenesis and morphogenesis in plants. However, it remains unclear how these signals transduct after CLE ligand binging in the shoot apical meristem. Now, we screened a novel gene AP2C1. ap2c1 mutant partly suppressed the carpel number in ap2c1crn-1 double mutant plants. To this end, genetic, molecular biological and cell biological approaches will be used to analyze the function of AP2C1, confirming that the ap2c1 mutant was responsible for the suppression of crn-1. Results from this study will for the first time elucidate the molecular and cellular mechanisms of AP2C1 in CLE signaling pathway, which will facilitate further understanding of the intracellular signal relay initiated by CLE ligand binding. This study will reveals another function of this MAPK cascade downstream of the CRN/CLV2 in regulating of stem cell homeostasis in shoot apical meristem.

植物大多数器官的形成是通过胚后发育而来，各个组织器官分生组织的干细胞是植物生长，产生新的组织和器官的源泉。植物多肽激素CLE (CLAVATA3/Embryo surrounding region-related) 对于维持植物干细胞动态平衡，器官发生及形态建成具有关键的调控作用。目前，针对于CLE信号传导途径中精密的传导步骤尚不清楚，尤其是CLV2/CRN如何传递信号的分子机制尚不清楚。申请人成功分离鉴定CLE信号传导途径的新因子AP2C1，ap2c1抑制crn-1的多心皮的表型。本项目以拟南芥茎顶端分生组织为研究对象，综合应用遗传学、分子生物学、细胞生物学等手段，解析AP2C1在CLV途径中的位置和功能，从而进一步阐述和完善CLE多肽激素通过MAPK信号级联反应参与调控植物分生组织干细胞动态平衡的分子机制。