马蹄莲对Pcc引起的软腐病抗性分子机制研究

The colored Zantedeschia is a very important new flower. However, production of the colored Zantedeschia is affected severely with soft rot caused by Pectobacterium carotovora subsp. Carotovora (called as Erwinia carotovora subsp. Carotovora before). Among the conventional ways to control soft rot: biological control, chemical control and cultivation measures, none is enough efficient. The ways based on plant resistance mechanism will be more efficient and direct, but the resistance mechanism of Zantedeschia spp. to soft rot is unknown. The 156 genetics with different resistance were collected in the previuos work. In this study, the influence of the pathagen Pcc on plant cell constitutive and plant growth, and Pathogen propagation/infection in plant after inoculation will be used as the efficient envidence to evaluate the phenotype resistance in Zantedeschia. The genes involving in the plant resistance will be marked and selected by association analysis combined the molecular markers from EST-SSR, NBS Profiling, and the phenotype. The whole sequence of 2-3 key genes will cloned by RACE, and identified their functions via expressing and/or siliencing them in the plants initially. All data will be used to explore the molecular mechanism of defense and resistance to soft rot in Zantedeschia, which will be valuable for making efficient control ways to soft rot and breeding new varieties with high resistance in the future.

彩色马蹄莲是世界重要的新兴花卉，但软腐果胶杆菌（Pectobacterium carotovora subsp. Carotovora，Pcc）引起的细菌性软腐病，对彩色马蹄莲生产造成了极大地的威胁，尚没有有效的防治方法。寄主的抗性机制是控制病害的重要依据，但目前有关马蹄莲软腐病抗性机制还远未明晰。本项目依托前期系统收集的高、中、低不同抗性的156个马蹄莲资源，以Pcc侵染对不同抗性马蹄莲生长和细胞学的影响、及不同抗性马蹄莲对侵染病原生长的影响为依据，鉴定不同马蹄莲资源的抗性；通过与EST-SSR、NBS Profiling分子标记进行关联分析挖掘软腐病抗性相关基因；并利用RACE克隆2-3个抗性相关重要基因全长后，利用病毒介导的基因沉默和/或遗传转化基因表达进行基因功能的分析与初步验证，解析马蹄莲对Pcc引起的软腐病抗性的分子机制，以期为科学防治病害和抗性新品种培育提供理论依据。

彩色马蹄莲因其花型独特、花色艳丽、花期长等优点，深受广大消费者的喜爱，被誉为二十一世纪“花卉之星”。然而，软腐细菌Pcc引起的软腐病严重危害彩色马蹄莲生产及其产品品质，被称为彩色马蹄莲生产的‘癌症’，极大的限制了彩色马蹄莲产业的发展。目前，虽然已经有不少生物、化学和栽培方法防治措施，但都不能从根本上控制该病害。马蹄莲软腐病抗性分子机制是制定病害防治措施和培育抗性品种的重要基础，有关马蹄莲软腐病抗性机制目前还知之胜少。本项目依托前期系统收集的高、中、低不同抗性的150个马蹄莲资源，以Pcc侵染对不同抗性马蹄莲生长的影响、及不同抗性马蹄莲对侵染病原生长的影响为依据，鉴定不同马蹄莲资源的抗性；通过与EST-SSR、NBS Profiling分子标记、马蹄莲全基因组（包括叶绿体基因组）进行关联分析挖掘软腐病抗性相关基因；并利用全基因组数据库获得6个抗性相关重要基因，正在利用全基因关联分析（GWAS）、软腐病菌侵染转录组进一步挖掘抗性基因和基因功能，进一步解析马蹄莲对Pcc引起的软腐病抗性的分子机制，研究结果为科学防治病害和抗性新品种培育提供了理论依据和重要基因资源。