基于磁性分子印迹固相萃取和电催化还原脱溴的四溴双酚A高选择性富集检测方法研究

According to the demand of low concentration and high selectivity for toxic tetrabromobisphenol A (TBBPA) in water environment, a detection method integrating magnetic molecularly imprinted solid-phase extraction (m-MISPE) preconcentration and electroreductive debromination determination was investigated. A construction method of m-MISPE based on microfluidic chip was investigated to improve the regeneration of traditional SPE method. A highly efficient and renewable preconcentration method of TBBPA was obtained. The surface and interface characteristic of nanomaterials and the electroreductive debromination mechanism were investigated to improve the response signal and selectivity. A highly selective TBBPA detection method was obtained. The m-MISPE preconcentration and the electroreductive debromination determination were integrating into microfluidic chip to apply accurate and on-line determination of TBBPA method in water environment.

本项目面向水环境中痕量有毒有机污染物四溴双酚A的低浓度和高选择性在线检测需求，提出一种集成有磁性分子印迹固相萃取富集功能和电化学还原脱溴检测功能的痕量四溴双酚A检测方法。本项目通过研究基于微流控芯片的磁性分子印迹固相萃取界面构筑方法，改善传统固相萃取方法的再生性，获得一种高富集效率和可更新式的四溴双酚A富集方法；通过研究纳米复合材料的表界面特性、电催化还原脱溴机理，利用还原脱溴特性，提高检测的响应信号和特异性，获得四溴双酚A高选择性检测方法；结合基于微流控芯片的磁性分子印迹固相萃取和电催化还原脱溴检测，获得一种四溴双酚A的富集检测一体化微流控芯片，为水环境中四溴双酚A的准确、在线检测提供一种新的方法和途径。

本项目面向水环境中痕量有毒有机污染物四溴双酚A的低浓度和高选择性检测需求，提出一种集成有磁性分子印迹固相萃取富集功能和电化学检测功能的痕量四溴双酚A检测方法。本项目研究了磁性分子印迹固相萃取微球的制备方法和富集分离效果，发现磁性分子印迹微球可对TBBPA产生较好的富集效果，富集效率高达40倍，实现了四溴双酚A的高选择性富集分离；研究了金刚石电极对四溴双酚A的响应特性及表面更新检测方法，并研究了不同碳链烷基溴化铵对TBBPA检测的增敏效应，实现了四溴双酚A的低浓度检测；研究了铂黑修饰钯电极对TBBPA的催化还原响应特性，并研究了集成富集分离和检测芯片的制备方法；最后通过集成磁性分子印迹固相萃取方法和电化学检测方法，实现了50 ng/L四溴双酚A的检测，为水环境中四溴双酚A的准确、在线检测提供了一种新的方法和途径。本项目研究成果获得了一项授权发明专利，并发表了4篇科技论文，完成了研究目标。