聚集诱导发光颜色变化材料的设计制备和性能研究

In the recent 30 years, the fluorescent probe technique originated in the end of 19 century has become an indispensable visualization tool for the field of biological analysis, clinical analysis, environmental monitoring, etc. The properties of organic fluorescent probes are largely determined by their luminogens. The ratiometric probes showing luminescent color change have better performances (in terms of anti-interference ability and accurate quantitative detection) than intensometric counterparts. We recently found that a few compounds with π-A-π conjugation system have bright emission and the ability to change their luminescent color due to the space hindrance. In the project, with the aids of theoretical calculations, we intend to improve the luminescent efficiency of these compounds in both the high concentration solutions and the aggregates by structural optimization, providing a new material base for the construction of high efficiency fluorescent probes. Moreover, the factors that can affect the luminescent performance in solutions and aggregates would also be investigated by steady and transient state spectroscopy methods. The research works in this project have momentous significance for developing and extending the application of organic materials in fluorescent probes.

开创于一百多年前的荧光探针技术，在最近三十年有了突飞猛进的发展，已成为了生物分析、临床分析、环境监测等领域不可缺少甚至是唯一的可视化工具。有机荧光探针的性能在很大程度上由其结构中所包含的发光母体材料所决定。相比发光强度变化的有机探针，发光颜色变化的探针（比率型）具有较强抗干扰能力，在准确定量检测方面有着更好的表现。最近我们发现一些具有π-A-π结构化合物的发光颜色随空间阻碍的改变而呈现明显变化。在此基础上，本项目拟结合理论化学方法，通过结构修饰，改善这类化合物在高浓度或聚集态时的发光效率，致力于发展一类没有明显浓度猝灭效应的单一组分发光颜色变化材料，为构建高性能荧光探针提供新的物质基础。同时通过各类稳态、瞬态光谱手段，深入研究影响这类材料在单分散以及聚集状态下发光性能的因素，为高性能荧光探针母体结构的设计提供思路和依据。

开创于一百多年前的荧光探针技术，已成为了生物分析、临床分析、环境监测等领域不可缺少甚至是唯一的可视化工具。有机荧光探针的性能在很大程度上由其结构中所包含的发光母体材料所决定。发光颜色变化的探针（比率型）具有较强抗干扰能力，在准确定量检测方面有着更好的表现。我们先前的研究已经发现一些π-共轭三芳基硼化合物发光效率较高，且其溶液发光与聚集体发光颜色具有明显的差异，有利于构建比率型探针。目前本项目通过引入推拉电子基团，发展了四个新杜基硼化合物，可用于聚合物内自由体积或温度的荧光颜色传感。研究了一个具有代表性的三芳基硼化合物在溶液和聚集态发光行为的差异，阐明了其发光颜色变化的机制。为扩展体系，还研究了一个基于双键链接的萘四酰二亚胺（NDI）共轭聚合物 PNV的聚集诱导发光变色现象。本项目的实施加深了课题组对π-共轭三芳基硼化合物以及发光共轭高分子的结构与发光性能的认识，为高性能荧光探针母体结构的设计提供思路和依据。