基于Notch信号通路探讨益气养血法对骨髓造血机能的改善作用及机制

Myelosuppression is induced by a variety of reasons, often complicated by serious complications. Now it has no ideal treatments for that. In our previous studies, we found that Replenishing Qi and Blood Herbs—Shengyu Tang could promote production of the peripheral blood cell, increase the level of expression of hematopoietic growth factors, and inhibit the myelosuppression. In the previous studies, it had found that Notch signaling pathway plays an important role in bone marrow hematopoietic Proliferation. In this study, we take the Notch signaling pathway as a starting point, and use Realtime-PCR, Western, and other methods, to explore the effect and mechanism of the Replenishing Qi and Blood method for bone marrow hemopoiesis. That may provide more reliable evidence and mechanism for the treatment of myelosuppression with Chinese medicine, and favorable to development of new myelosuppression drugs.

骨髓抑制是由多种原因引起的造血机能障碍，易引起严重并发症。益气养血法是中医治疗骨髓抑制的常用方法，临床中对骨髓抑制有较好的疗效，我们前期研究证实益气养血代表方药—圣愈汤及其单体药物可促进骨髓抑制小鼠外周血细胞生成、提高造血生长因子的表达，明显降低化疗后骨髓抑制发生率。同时既往研究发现一些益气养血类药物可以上调Notch信号通路，而Notch信号通路对干细胞的自我更新和增殖起着重要作用。因此我们推测益气养血药可能是通过骨髓基质细胞上调Notch信号通路，从而引起骨髓造血干细胞的自我更新和增殖，进而提高机体的造血机能，本研究拟以Notch信号传导通路为切入点，采用Realtime-PCR、Western、流式细胞术、RNA干扰技术等方法，从宏观和微观上探讨益气养血法对骨髓造血功能的改善作用及具体机制，为中医药防治骨髓抑制提供更可靠的证据和药物机理，为新的治疗骨髓抑制药物的研发提供基础