玉米产量性状基因精细定位与配合力解析

Maize is an important feed, industrial, and food crop. The general combining ability (GCA) and special combining ability (SCA) have been applied in maize breeding. Inbred lines with high combining ability and the combining ability determination in early generation selection proved that the combining ability locus can be inherited, but the theoretical research on the genetic architecture is relatively less. In this study, the crosses between B73 ×Mo17 (IBM) Syn10 double haploid lines and tests were constructed for QTL mapping of maize yield related traits and the combining ability effects. The relationship between the QTLs for maize yield related traits and combining ability was characterized. Combined with the whole genome resequencing of DH group and high resolution genetic linkage map, QTLs/genes for yield related traits and combining ability will be isolated. Favorite haplotypes/alleles for these candidate genes will be validated by an association mapping population. These data provide the basis for illumination of yield combining ability and the yield relationship between inbred lines and hybrids.

玉米是重要的饲料、工业原料和粮食作物。一般配合力和特殊配合力在玉米杂交育种中具有重要应用价值。高配合力骨干自交系的选育和育种选系群体配合力早代测定的实践奠定了配合力位点可遗传的基础，但是对其遗传基础解析的理论研究相对较少。本研究利用B73×Mo17 (IBM) Syn10 双单倍体群体与4个测验种配制杂交组合，分析产量及相关性状配合力效应、配合力相关QTL及其与性状本身QTL的关系，结合双单倍体群体的全基因组重测序与高分辨率遗传连锁图谱进行产量性状QTL及其配合力位点的定位与克隆。利用关联分析群体及与4个测验种组配的测交群体验证和阐明候选基因的优异单体型在育种进程中的传递，为解析自交系产量与杂交种产量关系、产量配合力形成的遗传基础提供依据。

玉米是重要的饲料、工业原料和粮食作物，配合力在玉米杂交育种中具有重要应用价值。本项目利用掖478×齐319重组自交系群体及其测交群体、关联分析群体，分析产量及相关性状配合力效应、配合力相关QTL及其与性状本身QTL的关系，结合重组自交系群体的全基因组重测序与高分辨率遗传连锁图谱进行产量性状QTL及其配合力位点的定位。研究结果显示，性状自身的46个QTL中只有28.3%的位点能够在性状GCA效应中被重复检测到。在本研究鉴定的与性状以及配合力显著相关的QTL置信区间内含有在玉米中已克隆的或者在水稻中已克隆与调控农艺性状发育相关的同源基因。进一步解析这些功能基因及候选基因在不同条件及组合间的表达模式对于进一步解析配合力形成机理具有重要意义。