复合Cr(Ⅵ)还原菌群修复铬污染土壤分子机理研究

The microbial bioremediation technology is a hot topic for the field of remedying Cr(VI)-contaminated soil with its potentially technological superiority.This program aims to solve the problem that the reducing efficiency of Cr(VI)-reducing microbes is unstable during remedying Cr(VI)-contaminated soil by the microbes, due to multiple environmental factors. To explore the molecular mechanisms of reducing Cr(VI) and of the effect on it by the multiple environmental factors, the chromium metabolic pathways of P. phragmitetus BB are investigated by the methods of genomics and molecular biology. According to the study mentioned above, the compound Cr(VI)-reducing bacterial community orientated by P. phragmitetus BB is built. The laws of bacterial synergistic growth and the molecular mechanisms of synergistically remedying Cr(VI)-contaminated soil by the compound Cr(VI)-reducing bacterial community are revealed by the methods of metagenomics, proteomics and molecular biology in genetic and cellular level, to provide scientific evidences for applying the efficient and stable compound Cr(VI)-reducing bacterial community in remedying Cr(VI)-contaminated soil.

微生物修复技术是Cr(VI)污染土壤修复领域的潜在优势技术和研究热点。为了解决Cr(VI)污染土壤微生物还原修复因受多重环境因素制约而Cr(VI)还原效率不稳定的瓶颈问题，本项目拟通过基因组学和分子生物学的方法，研究P. phragmitetus BB的铬代谢途径，探究Cr(VI)还原分子机理及其环境因素影响机理；以此为基础，构建以P. phragmitetus BB为主的复合Cr(VI)还原修复菌落结构，运用宏基因组学、蛋白质组学和分子生物学的方法，在基因和细胞层面上阐明复合还原菌群协同生长规律和修复Cr(VI)污染土壤的协同作用分子机理，为高效、稳定的复合还原菌群在Cr(VI)污染土壤修复中的应用提供科学依据。

我国现阶段 Cr(VI)污染土壤情况十分严重。因此，对我国 Cr(VI)污染土壤进行合理有效的修复已经迫在眉睫。微生物修复技术是 Cr(VI)污染土壤修复领域的潜在优势技术和研究热点。针对单一微生物还原效率不稳定的瓶颈问题，本项目提出建立以Pannonibacter phragmitetus BB 为主，辅以Shewanella oneidensis MR-1和Pseudomonas putida F1的高效Cr(VI)还原菌群。围绕如何建立高效、稳定的复合Cr(VI)还原菌群，本项目揭示了Pannonibacter phragmitetus BB全基因序列，阐明了基因表达调控与Cr(VI)还原效率的关系，建立了高效高效Cr(VI)还原菌群复合方法和标准，开发了基于实时定量PCR的复合菌群基因协同调控研究方法，探究了复合菌群协同作用规律，明确了土壤理化性质与复合菌群基因协同调控的反馈机制，丰富了复合菌群还原Cr(VI)的理论，为高效、稳定的复合还原菌群在 Cr(VI)污染土壤修复中的应用提供科学依据。. 通过项目研究，发表SCI学术论文5篇；培养博士1名（毕业1名），硕士2名（转直攻博1人）。