具有高催化活性的压力响应型纳米复合探针的构建及其在循环肿瘤细胞快速便携式检测中的应用

In recent years, the incidence of cancer is increasing year by year , and cancer has been public enemy number one for human life and health. One of the important reasons for the high mortality of cancer is due to the lack of accurate detection methods for early diagnosis of cancer, and cause the proliferation and metastasis of tumor. Research shows that circulating tumor cells (CTCs) are related to the proliferation and metastasis of cancer closely. As the most valuable biomarker for cancer research, circulating tumor cells can act as the important evidence in the early diagnosis of tumor. Hence, the development of an effective method to detect the circulating tumor cells is crucial means for fighting cancer. Based on our previous work, we intend to construct a class of 3D magnetic nanoprobes with high catalytic activity for amine borane decomposition by using the coordination assembly method. Furthermore, these magnetic nanoprobes are further modified with targeting molecules by covalent bond. This method integrating of a catalyzed gas-generation reaction with a cancer recognition system leads to significant pressure changes, which can be measured with high sensitivity by using a low-cost and portable pressure meter. By virtue of its excellent selectivity and sensitivity, simple and low cost, this detection methodology could provide experimental basis and theoretical references for the incipient cancer diagnosis.

近年来，癌症发病率逐年攀升，已成为危害人类生命健康的头号公敌。而导致癌症死亡率居高不下的一个重要原因就是无法实现癌症的早期准确诊断，导致肿瘤的扩散和转移。研究表明，循环肿瘤细胞与癌症的扩散和转移密切相关，被视为肿瘤转移极具标志价值的生物标志物，可作为癌症早期诊断的重要依据。因此，发展一种行之有效的方法来检测血液中的循环肿瘤细胞是抗击癌症的重要手段。本项目拟在前期工作基础上，利用配位自组装的方法构建一系列三维磁性纳米复合探针，这些探针具有大的比表面积和丰富的孔洞结构，可以高效催化氨基硼烷分解产生氢气。此外，磁性纳米探针表面共价修饰的癌细胞靶向分子，可以成功实现催化产气反应与循环肿瘤细胞检测系统的对接，以压力作为输出信号，利用廉价、便携的气压计便可实现血液中循环肿瘤细胞的实时快速检测。该检测方法选择性好、灵敏度高、操作简单、成本低廉，为癌症的早期诊断提供了一定的实验基础和理论依据。

近年来，随着生活水平的不断提高，人们身体素质却逐渐恶化，这主要归因于食品安全和环境污染问题对人类的危害。研究表明，人类诸多疾病的发生与体内一些重要生物标志物及离子的含量有关，因此，构建一种简单、快速、便捷的分析新方法用于疾病相关标志物的检测对于医疗诊断及健康管理具有重要意义。. 本项目以二维材料、金属有机框架材料、过渡金属配位聚合物、贵金属、金属氧化物等纳米材料为基础，制备了一系列具有特殊微纳界面结构的功能复合材料。得益于这些功能复合材料的高催化活性，可将其成功应用于构建便携式传感平台。深入研究了材料结构与其催化活性之间的关系，阐明了利用催化反应实现被检测物输出信号产生及放大的机理，建立了一系列便携式检测生物标志物及离子的新方法。以上项目研究为构建高灵敏度、高选择性的便携式传感平台提供了一定的理论依据和实验基础。