光敏DNA纳米管载体自组装及其光控释放性能

Self-assembled DNA nanostructures are among the most promising candidates to serve as medical nano-carriers, which benefit from the high level of structural programmability, spatial orientation, precisely targeting ligands, biocompatibility, biodegradation and water solubility. .On the base of DNA nanotubes assembled by circular DNA, photo-responsive DNA nanotube (PR-DNA-NT) can be developed by inserting azobenzene into the DNA blocks. The formation and dissociation of azobenzene-inserted-DNA duplex could be simply switched through the cis-trans isomerization of azobenzene by light irradiation, so is the structure of PR-DNA-NT. As medical carriers, the load efficiency, stability and light controlled release property of PR-DNA-NT will be estimated further..Photo-responsive DNA nanotube have several advantages such as density assembly, multi targeting ligands and avoiding complex design as normal DNA origami, and can be stimulated by clear, noninvasive and efficient light source, which endorse PR-DNA-NT as a versatile nano-carrier.

与常规纳米载体相比，DNA自组装纳米结构尺寸、空间指向性以及表面功能化单元可以精确控制，且具有天然的生物相容性、可降解性和水溶性等特点，由此DNA纳米结构成为最有前途的纳米载体之一。. 本项目拟在环状DNA自组装纳米管基础上，设计偶氮苯掺杂DNA序列，组装光敏DNA纳米管。通过DNA序列中偶氮苯官能团的顺反异构，光控掺杂DNA与其天然互补链杂交和解链，从而实现光敏DNA纳米管光致结构转变。进一步考察光敏DNA纳米管结构作为载体的稳定性和负载效果，研究其光控释放性能。. 光敏DNA纳米管既具有DNA折纸纳米结构的紧密堆积、多位点负载等特点，又可避免常规折纸体系对序列设计的复杂要求，同时实现洁净、无创、高效的光刺激响应，有望成为一种强大的多功能载体。

DNA纳米材料是一类新的功能材料，可以在纳米尺度“自下而上”地组装成各种设定形状、大小等特定空间可寻址性和可扩展性分子器件和纳米装置。本项目利用偶氮苯修饰DNA组装得到光响应纳米条带、纳米管、二维平面等光响应tile类无限结构，以及二维SST、二维origami等光响应有限结构。进一步通过调节照射波长、光强和照射时间，可逆或不可逆地调节各种纳米装置的大小、形状、构象。这些光响应DNA纳米结构在生物学、生物医学、材料科学、纳米光子和纳米电子等领域具有深远的应用潜力，同时也为光控DNA系统所面临的挑战和进一步的发展提供基础支持。