激素处理结合变温层积对大百合种胚形态后熟的影响及机理研究

Cardiocrinum giganteum（Liliaceae） is one important resource plant. Due to the morphological-physiological dormancy（MPD）, the seeds can only germinate after a long period of embryo postmaturation, which is a great obstacle for sowing and propagation. The method of changing temperature stratification combined with exogenous hormone treatment is an effective way to solve the problem. However, the mechanism of seedembryo development is unclear, especially the study in the molecular mechanism of seedembryo development is still blank. In this study, on the basis of a previous research,the seeds of Cardiocrinum giganteum were treated with exogenous hormones before changing temperature stratification and the optimum scheme of the treatment of exogenous hormones was screened. Then,the relationship between development progress of seedembryo (ratio of embryo) and the dynamic changes of endogenous hormones was analyzed during changing temperature stratification. Combined with the study in transcriptome expression in the control, we try to clarify the main physiological reaction and the related genes. The utilization of morphology, physiology and methods of molecular biology perspectives can provide theoretical and practical guidance for the propagation of seedlings of Cardiocrinum giganteum.

大百合（Cardiocrinum giganteum）是百合科（Liliaceae）大百合属植物，具有独特的观赏性和较高营养价值，极具开发利用潜力。前期的研究表明，其种子存在形态—生理休眠（MPD），自然休眠期长达18个月，对于种苗生产极其不利；激素处理结合变温层积是促进胚后熟发育并解除种子休眠的有效途径。但是，大百合胚的后熟发育机理尚不清楚，尤其缺乏分子机理研究的资料。本研究以申请者多年的前期工作为基础，以大百合种子为材料，优化变温层积中添加外源激素的试验方案；基于优化方案，采用形态学、生理学和分子生物学的方法和手段，通过分析变温层积中大百合种胚发育进程与生理指标变化的相关性，结合变温层积过程中的转录组表达谱，阐明变温层积中添加外源激素对大百合种胚的生理影响及相关基因的表达特征。研究结果能系统、深入地揭示大百合种胚后熟发育的生理机制，并首次为大百合种胚后熟发育的机理研究提供分子层面的依据

本研究以以大百合种子为材料，筛选添加外源激素并结合变温层积的优化试验方案，5 mg/L 2,4-D 浸泡大百合种子48 h后再进行变温层积有利于促进胚的生长并缩短萌发时间；基于优化的外源激素添加方案，采用形态学、生理学和分子生物学的方法和手段，分析变温层积中大百合种胚发育进程与内源激素变化的相关性，结合变温层积过程中的转录组表达谱，阐明添加外源激素并结合变温层积对大百合种胚的生理影响及相关基因的表达特征。研究结果能为大百合种胚后熟发育的机理研究提供生理和分子层面的依据。本项目资助发表论文2篇，带发表2篇；申报发明专利1项；培养硕士研究生2名。