DNA损伤诱发Ndrg2核转位的机制和功能研究
基本信息
结项摘要
细胞质与细胞核之间存在活跃的物质交换，部分蛋白质通过细胞质与细胞核的穿梭发挥其生物学功能。NDRG2是本研究室最先发现的一个新基因，该基因是NDRG家族成员之一。本课题组研究发现DNA损伤可以诱发Ndrg2核转位，但其具体机制以及生物学意义有待于进一步研究。我们拟通过截短突变体研究Ndrg2核转位的结构基础；体外核转位实验观察Ndrg2核转位是否依赖核转运蛋白；通过调控蛋白激酶及精氨酸甲基转移酶的活性观察其核转位与磷酸化、甲基化的关系；通过观察DNA损伤条件下相关分子的变化与Ndrg2核转位的时效关系,寻找可能的调控因子；融合表达核定位信号与核输出信号调控Ndrg2亚细胞定位,观察Ndrg2核转位在DNA损伤应激反应中的功能。本研究从上述几个角度进行探讨,将对DNA损伤刺激条件下Ndrg2核转位的机制以及生物学意义予以初步阐明。
项目摘要
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数据更新时间：2023-05-31
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