我国新发现的两种牛泰勒虫的生物学特性及分类的研究

Identification of a species of Theileria isolated from cattle in central parts of Gansu province. Morphological comparison with T.annulata、T.sergenti、T.parva、T.mutans、T.tautotragi、T.velifera、T.buffeli suggested that the species of Theileria not only have the shape shared by Theileria, but also some strange shapes beyond word, especially when parasitemia was high, the rate of the strange shapes could be as high as 20%. The budding multiplication was observed. The parasites could proliferate in splenectomized cattle and parasitemia were as high as 52.69%，and the infected cattle showed serious anemia, high temperature listless, lack of the appetite, some of the cattle died of theileriosis. Damages in some organs were observed in postmortem examination. Using Theileria specific primer, the genomic DNA of the new Theileria could be amplified, which suggested that the parasite is Theileria. The 18s small subunit ribosomal RNA gene of the parasites was sequenced and compared with that of 9 species of Babesia and 7 species of Theileria. The results indicated that the new species of Theileria was different from these valid Babesia and Theileria. The parasite was close to T.buffeli and T.sergenti, and the difference with T.mutans, T.tautotragi, T.annulata, T.velifera and T.parva was obvious. As for the results mentioned above, the parasites was considered to be a new species of Theileria. The parasites were named Theileria sinensis due to it was firstly isolated in China..The experiments demonstrated that the vectors of Theileria sinensis were H.qinhaiensis and H.japonica. Inoculation experiments, morphological observations, transmission experiments, and sequencing of 18S rRNA sequences were conducted with a species of T.sp isolated from yaks on Weiyuan ranch of Gansu province. The results showed that T.sp and T.sinensis belong to the same species.

确定牛泰勒虫一新种的媒介蜱和传播方式；观测临床症状、血液学和病理变化；进行流行病学调查;对药物敏感性和宿主特异性试验，提出防制措施，最后完成新种的命名工作。完成Ｌ├粘嫖炊ㄖ值拇糠掷耄票窪NA，进行与分类有关的基因序列分析和PCR 扩增试验,确定弥值姆掷嗟匚弧⒉≡匦浴⒚浇轵绾痛シ绞降取１鞠钛芯烤哂屑渲匾睦砺垩跫壑岛颓痹诘木靡庖濉