布鲁塞尔德克酵母核小体图谱与有氧发酵途径的进化

Dekkera bruxellensisis a highly potential exploitation strain for alcohol fermentation. The species diverged from the budding yeast branch about 200 million years ago, and independently evolved aerobic fermentation pathway. Since aerobic fermentation would accelerate cell growth under aerobic condition by fermentative process, we speculated that the optimization of this process would be a key step to reform Dekkera bruxellensis strain for alcohol fermentation. Nucleosome usually plays an important role in the evolutionary process. In this proposal, we would focus on studying the function of nucleosome during the evolution of aerobic fermentation in Dekkera bruxellensis strain. At first, we proposed to identify the difference of transcriptome and nucleosome profile in Dekkera bruxellensis strainand it's relative. Secondly, we would figure out the relationship of nucleosome profile and gene expression variation in both strains. At last, we proposed to decode the function of nucleosome profile during the evolutionary process of aerobic fermentation in Dekkera bruxellensis strain. At the same time, we would modify some important genes in aerobic fermentation pathway to improve the fermentative efficiency of Dekkera bruxellensis strain. Our results would exhibit the function of nucleosome profile during the evolutionary process of aerobic fermentation, and also provide important theoretical supports for reformation of Dekkera bruxellensis strain in fermentative industry.

布鲁塞尔德克酵母是极具工业开发潜力的菌种，早在2亿年前就已经同酿酒酵母分离并且独立进化出了有氧发酵途径。由于有氧发酵途径可以使细胞在有氧条件下利用发酵途径快速生长，因此有氧发酵途径的优化是布鲁塞尔德克菌种工业化改造的关键步骤。核小体参与的表观遗传是生物遗传变异的重要分子机制之一，本项目将集中研究核小体在布鲁塞尔德克酵母有氧发酵途径进化过程中的作用。首先我们将比较研究布鲁塞尔德克酵母及其近缘物种的转录组和核小体图谱。其次，我们将结合转录组和核小体图谱分析核小体分布的进化差异对基因表达的影响。最后，我们将解析核小体图谱与有氧发酵途径进化的相关性，并对部分重要基因的核小体分布状态进行改造，从而提高布鲁塞尔德克酵母的发酵效率。研究结果将揭示核小体图谱在有氧发酵途径进化过程中的意义，同时为布鲁塞尔德克酵母的工业化改造提供重要理论依据。