基于纳阵列结构的样品富集与检测器件研究

It is a very important significance in the filed of disease prevention, clinical diagnosis and disease treatment to develop new methods for efficient sample enrichment and highly sensitive detection. Microfluidic technology for biomarkers enrichment and detection has become an important research topic in analytical science. The research and development of microfluidic-based nanoarray devices and the development of new enrichment and detection methods will improve the enrichment efficiency and analytical sensitivity. We will start this project from the design and preparation of functionalized nanoarray devices including research of new device processing techniques and controlled functionalization methods. The new theroy on metal enhanced fluorescence, surface enhanced Raman, MADLI-MS and electrocatalysis based on the nanoarray devices will be studied. To achieve analysis of clinical samples, we will study and develop new efficient enrichment and high sensitive detection methods for low-abundance biomarkers. In addition, the nanoarray devices will further integrated into microfluidic systems for automaticly efficient enrichment and highly sensitive detection of tumor related proteins and cells.

用于低丰度生物标志物富集与检测的微流控技术已经成为分析科学的重要研究前沿内容之一，发展新的高效富集方法和高灵敏检测微纳器件对于疾病的预防、临床诊断及疾病治疗等具有非常重要的意义。将微流控方法与纳阵列器件研究相结合、发展相应的新的富集和检测策略是实现微量样品富集效率和分析检测灵敏度提高的重要途径。本课题从设计和制备功能化的纳阵列器件出发，在发展新的器件加工技术和实现微纳器件功能化方面开展研究。研究并探索基于纳阵列结构的金属增强荧光、表面增强拉曼、基质辅助激光解析生物质谱和电催化的检测新原理。在此基础上，研究并建立新的高效富集和高灵敏检测方法，并应用于微量临床样品的分析和检测，研制出集成有纳阵列核心器件的集成化微流控系统，实现对肿瘤诊断相关的蛋白质和细胞的自动化、高效富集和高灵敏检测，开拓生物分子富集和检测的新途径。

本课题从纳阵列器件的制备和功能化出发，研究了微流控体系对生物分子识别、富集的影响，开展了微孔阵列芯片对肿瘤细胞的分离，捕获和成像工作。首先，我们制备了具有微孔阵列的芯片，合成了包括纳米银、二硫化钼等材料，并且实现了微阵列芯片的功能化。其次，我们对微流控芯片进行改进，研究了微流控体系下生物分子富集过程，根据荧光信号的变化去追踪分子富集过程中的变化。另外，我们还研究了微流控体系对单颗粒成像的影响，建立了目标分子的检测模型。最后，我们结合微纳阵列器件和微流控体系，实现了单细胞水平肿瘤细胞的捕获和成像工作。