可封闭、自供应纳米电化学体系构建及超敏分析检测研究

The project is proposed to aim at the key issue and urgency requirement on device simplification and model innovation in nano-electrochemistry field, set artificial nano-channel as the substrate, use facile ion sputtering method to fabricate the micro-device of nano-channel and nano-electrode, create liquid/liquid transfer method to introduce liquid interface on two sides of nano-channel, in turn build sealable nano-electrochemistry system; based on the proposed system, investigate the electrochemical behaviors and signal-readout ways of redox cycling and ion-channel current that based on the nano-channel and nano-confinement effects, introduce highly efficient bio-/nano-catalysis and investigate their electrochemical behaviors, create novel electrochemical self-supplying model, build ultra-sensitive electrochemical analysis and biosensing models based on the synergetic enhancements deriving from the bio-/nano-catalysis, sealable nano-electrochemistry systems and electrochemical modulation, in turn apply in the sensitive detection of hazards of food safety. Therefore, the project is planned to focus on (1) the construction and electrochemical behavior of sealable nano-electrochemistry system, (2) development of sealable nano-electrochemistry models combining with bio-/nano-catalysis, (3) ultra-sensitive analysis and biosensing for food safety.

本项目拟针对纳米电化学中装置简化、模式创新的核心问题和迫切需要，以人工纳米通道为平台，采用离子溅射法创制纳米通道两侧喷镀导电层（电极）的纳米电化学装置，基于该装置开发两相转移法，在纳米通道两侧形成相界面，籍此构建封闭纳米电化学体系；基于该体系，探索基于纳米限域效应和纳米通道效应的氧化还原循环、离子电流等电化学行为和信号输出方式，引入高效酶催化和纳米催化反应并研究其在封闭纳米体系中的电化学响应行为，基于电化学合成开发自供应新方法，构建基于封闭纳米体系、生物/纳米催化和电化学调制协同增强信号的超敏甚至单分子纳米电化学分析和生物传感新模式，用于食品安全对象的灵敏检测，具有创新性和应用前景。因此，本项目主要研究（1）可封闭纳米电化学体系的构建与电化学行为；（2）结合高效生物/纳米催化的新型封闭纳米电化学模式；（3）食品安全超敏分析检测应用。

本项目开展了基于纳米通道和纳米限域效应的超灵敏分析检测方法研究，创制了纳米电化学芯片电极和反应装置，开发了基于纳米通道和液膜的封闭纳米电化学体系，引入生物/纳米催化提出了基于纳米通道效应和纳米限域效应的电化学分析检测技术，探索了限域空间下生物和化学反应行为和机制，建立了5种基于纳米限域的分析和检测新模式；提出了3种基于金属有机框架薄膜限域吸附的样本前处理方法，创制了多种薄膜器件，实现了复杂样本中目标物的快速和高效收集；开发了系列简便、快速和灵敏的分析和检测技术，用于致病微生物（细菌和病毒）和抗生素等的检测性能满意，并应用于食品安全检测。共发表SCI论文16篇，其中美国化学会和影响因子大于10的论文10篇，封面论文2篇；授权专利3件；出版英文专著章节1节；培养研究生10名，其中毕业6名。