可激活荧光活体成像用于黄曲霉毒素体内代谢分布的实时无损研究

Development of real-time and in situ analytical methods for sensitive and selective determination of food and environment-borne hazardous substances in-taken into human body, such as toxins or pathogens, appears as the key research direction in the environment pollution and food safety area, so as to clarify the harmful action mechanism inside the human body through in situ collecting the information of their in vivo behaviors. Herein, optical imaging technology with real-time monitoring and non-damage detection ability was introduced for specific recognition of aflatoxin in cellular levels and in vivo via the fluorescence resonance energy transfer (FRET) protocol. Persistent luminescence nanophosphors (PLNPs) that possess the instinct advantages of improved signal-to-noise ratio and sensitivity of imaging, as well as the excellent photostability and biocompatibility, were employed as the photoluminescence nanoprobes, while the copper sulfide nanoparticles (CuS) were utilized as the quencher. This work will open up the new way for the applications of bioimaging in food safety detection, and provide the advisory roadmap of bioimaging-guided investigation and mediation of food and environment-borne contamination effect to human health.

明确环境和食品中的危害物质在体内的代谢转化过程和危害作用机理是环境污染与食品安全的重点研究方向，而发展实时无损的分析方法对于揭示环境污染和食品安全与人类健康的科学关系具有重要意义。本项目瞄准生物毒素的环境污染与食品安全这一前沿热点，以黄曲霉毒素AFT B1为研究对象，构建以长余辉纳米材料(PLNPs)为发光中心、硫化铜纳米粒子(CuS)为猝灭剂的复合纳米探针，通过二者之间的荧光共振能量转移过程和适配体的选择性识别，建立AFT B1的特异性检测方法，进而借助近红外PLNPs可体外激发及优异的生物相容性和光学稳定性，进一步实现PLNPs-CuS复合纳米探针在活体内的选择性识别AFT B1的荧光成像技术，实时无损的原位监测AFT B1在生物体内的代谢分布行为。本项目旨在首次将活体成像技术的实时监控和无损检测优势引入环境科学与食品科学研究领域中，有望拓展创新性的环境污染和食品安全研究理念。

明确环境和食品中的危害物质在体内的代谢转化过程和危害作用机理是环境污染与食品安全的重点研究方向，而发展实时无损的分析方法对于揭示环境污染和食品安全与人类健康的科学关系具有重要意义。本项目围绕生物毒素的环境污染与食品安全问题，以黄曲霉毒素AFT B1为主要研究对象，构建了以长余辉纳米材料(PLNPs)为发光中心、硫化铜纳米粒子(CuS)为猝灭剂的复合纳米探针，通过二者之间的荧光共振能量转移过程和适配体的选择性识别，建立了AFT B1的特异性检测方法，进而借助近红外PLNPs可体外激发及优异的生物相容性和光学稳定性，进一步实现了PLNPs-CuS复合纳米探针在活体内的选择性识别AFT B1的荧光成像技术，实时无损的原位监测AFT B1在生物体内的代谢分布行为。本项目主要创新在于首次将活体成像技术的实时监控和无损检测优势引入到环境科学与食品科学研究领域中，拓展了一种创新性的环境污染和食品安全研究理念。