基于五种功效的大黄药对配伍机制及其共性关系研究

Radix et Rhizoma Rhei, the major usage of which are purgation, purging fire, heat-clearing and detoxifying, activating blood and removing stasis, alleviate water retention, are widely used in clinical therapy in different prescription. These five efficacies of Radix et Rhizoma Rhei not only correspond with the symptom of patients's disease but also closely relate to the compatibility of herbs. The present study of compatibility mechanism research of Radix et Rhizoma Rhei were isolated and not simultaneously that only focused on single efficacy and relate chemic ingredients which can't explain the common relationship and correlation within different campatibilities. Five of Radix et Rhizoma Rhei pair herbs and Aurantii Fructus Immaturus, Radix et Rhizoma Rhei and Coptidis Rhizoma, Radix et Rhizoma Rhei and Moutan Cortex, Radix et Rhizoma Rhei and Persicae Semen, Radix et Rhizoma Rhei and Kansui Radix that derived from Dachengqi decoction, Xiexin Decoction, Dahuangmudan decoction, Taohechengqi decoction and Daxianxiong decoction respectively, are selected as objects for experimental conduct of pharmacology, pharmacokinetic and serum pharmacochemistry. In order to explain the mechanisms of compatibility of Radix et Rhizoma Rhei, experimental data will be analyze by mathematical statistics as to studying the correlation between ingredients and efficacy through the perspective of five-efficacy simultaneously. This project is going to make sense for the research of traditional Chinese medical theory through multidiscipline, and provide the experimental reference for preparation extraction technology and clinical medication of Radix et Rhizoma Rhei compatibility.

大黄在临床的主要用法有泻下、泻火、清热解毒、活血袪瘀和利水，见于多种复方配伍中。这五种不同方向的功效作用，除了与治疗疾病症候的不同产生差异外，与大黄的配伍作用关系密不可分。目前对大黄配伍的研究，均是对单独的配伍功效、药效和化学成分进行研究，是独立的、单指标的、非同步的，未能反映大黄在不同配伍当中体现的共性关系和各项指标的相关性。本项目拟选取大黄经典组方"大承气汤、泻心汤、大黄牡丹汤、桃核承气汤和大陷胸汤"中的药对"大黄枳实、大黄黄连、大黄牡丹、大黄桃仁和大黄甘遂"进行药理学、药代动力学和血清药物化学实验研究，采用数理统计方法分析数据，从大黄五种功效角度，同步、整体、系统地探索配伍药物"体外成分--体内成分--药效"之间共性关系及相互影响的过程，揭示大黄药对配伍作用机制及规律。此研究对于多学科理论探讨中医药理论具有积极意义，同时为大黄复方及其制剂提取工艺的设计、改进和临床用药提供实验依据。

大黄在临床的主要用法有泻下、泻火、清热解毒、活血袪瘀和利水，见于多种复方配伍中。这五种不同方向的功效作用，除了与治疗疾病症候的不同产生差异外，与大黄的配伍作用关系密不可分。目前对大黄配伍的研究，均是对单独的配伍功效、药效和化学成分进行研究，是独立的、单指标的、非同步的，未能反映大黄在不同配伍当中体现的共性关系和各项指标的相关性。本项目拟选取大黄经典组方“大承气汤、泻心汤、大黄牡丹汤、桃核承气汤和大陷胸汤”中的药对“大黄枳实、大黄黄连、大黄牡丹、大黄桃仁和大黄甘遂”进行药理学、药代动力学和血清药物化学实验研究，采用数理统计方法分析数据，从大黄五种功效角度，同步、整体、系统地探索配伍药物“体外成分--体内成分--药效”之间共性关系及相互影响的过程，揭示大黄药对配伍作用机制及规律。此研究对于多学科理论探讨中医药理论具有积极意义，同时为大黄复方及其制剂提取工艺的设计、改进和临床用药提供实验依据。