褪黑素通过mTOR信号通路介导的自噬在脊髓损伤后的保护作用及机制研究

Melatonin has important physiological and pathological functions, it can regulate the body's circadian rhythm and seasonal. As a reducing agent, melatonin have an effect of scavenging free radicals and anti-oxidationas. In recent years, some studies have reported that melatonin had a protective effect on spinal cord injury (SCI). Our previous study has found that: the mechanism of melatonin on protect SCI may be related to regulate autophagy. This study was to observe the effect by adjusting the mammalian target of rapamycin (mTOR) signaling pathway after SCI, and detect nerve cells apoptosis, verify the role of promoting the recovery of neurological function. Ultimately, the study will confirm that the nerve protective effect of melatonin is by adjusting autophagy via mTOR signaling pathway and provide a theoretical basis and experimental evidence for the clinical treatment by melatonin after SCI.

褪黑素具有重要的生理和病理功能，能够调节人体的昼夜节律性和季节节律性，可以作为还原剂起到清除自由基、抗氧化的作用。近年来研究发现褪黑素对脊髓损伤（SCI）有保护作用。我们前期研究发现：褪黑素保护SCI的机制可能与调节自噬有关，本实验拟通过观察SCI后褪黑素对雷帕霉素靶蛋白mTOR介导的自噬的调节作用，同时检测神经细胞凋亡情况、验证促进神经功能的恢复作用等，最终确认SCI后褪黑素的神经保护性作用是通过调节mTOR信号通路介导的自噬而实现，为褪黑素临床治疗SCI提供理论基础与实验依据。

褪黑素具有重要的生理和病理功能，能够调节人体的昼夜节律性和季节节律性，可以作为还原剂起到清除自由基、抗氧化的作用。近年来研究发现褪黑素对脊髓损伤（SCI）有保护作用。我们前期研究发现：褪黑素保护SCI的机制可能与调节自噬有关，本实验通过观察SCI后褪黑素对雷帕霉素靶蛋白mTOR介导的自噬的调节作用，同时检测神经细胞凋亡情况、验证促进神经功能的恢复作用等，最终确认SCI后褪黑素的神经保护性作用是通过调节mTOR信号通路介导的自噬而实现，为褪黑素临床治疗SCI提供理论基础与实验依据。