质粒ＤＮＡ特殊拓朴结构实体的研究
基本信息
结项摘要
本项目以细菌细胞中抽提的质粒DNA为研究对象，研究特殊DNA拓扑结构。应用凝胶电泳、密度梯度超离心、电子显微镜学及扫描遂道显微法研究DNA拓扑结构实体的物理、化学及结构特性。发现a.游离PUC18DNA超螺旋分子具有线圈型结构，为中空球形颗粒，平均外直径43.8nm，包装比21。发现松驰环形DNA分子也存在线圈型结构，其外直径53.1nm。b.质粒DNA分子内存在特殊的拓扑键，可把分子分隔出花瓣样小环。这种拓扑键可能是把细胞染色体分隔成环的结构因子。c.扫描遂道显微法证实，质质粒pUC18DNA可形成面包圈状超凝集结构，外直径16nm。以上结果是DNA结构研究的最新进展，有重要学术价值。
项目摘要
项目成果
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数据更新时间：2023-05-31
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