新型BODIPY类纳米荧光探针的制备和性质研究

Fluorescent probes will be designed and synthesized based on 4-bora-3a,4a-diaza-s -indacene (BODIPY) derivatives as fluorophores due to their excellent fluorescence properties. New nano fluorescence probes were obtained when theses BODIPY probes were futher covalently attaches and immobilize onto the surface of biocompatible hydroxyapatite (HA) nanoparticles or silica nanoparticles. This new kind of nano fluorescence probes contains the intrinsic properties of both such as good solubility, low toxicity and high biocompatibility，robustness against light and chemicals, excitation/emission wavelengths in the visible to near infrared spectral region (500-800 nm). The difference properties between them and organic fluorescent dyes or other nano fluorescence probes will be investigated. The research will get some new finding in the study from design, preparation and investigate between the structures of the probes and their desired spectroscopic and photophysical characteristics. The study will development of a new way to the design and synthesis of new BODIPY-based biocompatible nano fluorescence probes for the study used in biomedicine as well as for fluorescence microscopic imaging in the future.

拟以具有优良荧光性能的硼-二吡咯亚甲基(BODIPY)作为发光基团合成BODIPY类荧光探针，将其同水溶性好，毒性低，生物活性及生物相容性好的纳米羟基磷灰石或纳米二氧化硅通过共价键合牢固固定在纳米颗粒的表面上获得两者优点兼具的,同时还具有高度光稳定性的,荧光的激发和发射波长覆盖了从可见光到近红外光范围(500-800 nm)的新型BODIPY类纳米荧光探针。系统的研究和比较该类探针同传统有机荧光探针以及其它类型纳米荧光探针在各种性能上的区别，探索性能与结构之间的关系。整个项目从纳米荧光探针的设计，制备，结合性能和激发态光物理光化学性质的研究将会有新的发现，项目结束后所获得的研究成果对生物相容性高的纳米荧光探针的设计和制备，以及在生物学和造影成像研究具有重大的价值。

BODIPY类荧光染料由于具有摩尔吸光系数大，荧光量子产率高，荧光峰的半峰宽狭窄，激发和发射波长处于可见光区，光化学性质稳定性高的优点，一直以来被作为基本的发光基团用于设计和发展新型荧光探针。尤其最重要的是，人们可以根据自己研究所需，通过对它的适当的位置加以化学修饰来合成符合实验要求的具有不同化学和光谱学性质的化合物。本研究工作一个方面是以基于BODIPY来设计合成可用于生物成像检测pH值，重要生命金属离子和生物分子的荧光传感器。.. 荧光碳量子点因其优秀的的光学特性成为了人们研究人员的研究热点，如粒径尺寸小、激发波长范围宽、发射波长可调节、荧光效率高、光稳定、易实现表面功能化等这些特点使其应用更加广泛。与传统的量子点相比，碳点因其良好的生物相容性和低毒性，在荧光生物探针和生物成像方面也有了很大的进展。本研究中，我们通过表面修饰使碳点功能化形成碳点复合材料， 系统的研究复合材料形貌，结构，发光和催化性能等性质，同时试图揭示碳量子点发光机理。