贮存蛋白基因在家蚕抵御白僵菌感染过程中的作用机制研究

Beauveria bassiana is a kind of important pathogenic fungi to silkworm and other economic insect, and it is also widely used as biological insecticides in controlling of agricultural and forest pests. Till now, few antimicrobial peptides and proteins with antifungal activity have been discovered in insects. In this research, we will use silkworm storage protein genes as the research object, clone and expression these genes, then study its model of regulation, and screen the genes interact with them, the interaction between storage protein and B. bassiana in vivo and in vitro of silkworm. We also will study the effect of storage protein genes during the melanization reaction in silkworm. Based on these results, we will analyze the mechanism of silkworm storage protein against B. bassiana. These results will provide reference for the mechanism of B. bassiana infection and pests biological and genetic control.

白僵菌是家蚕等经济昆虫的重要病原真菌，也是农林害虫生物防治中常用的生物杀虫剂之一。目前在昆虫中发现的具有抗真菌作用的抗菌肽或抗菌蛋白很少，本项目拟在国家自然科学基金项目（31272498）研究的基础上，以筛选、获得的家蚕贮存蛋白基因为研究对象，通过克隆、表达贮存蛋白，研究其表达调控模式和与之互作的基因，及其与白僵菌在家蚕体内、体外的相互作用关系，研究贮存蛋白在黑化反应中的作用，分析其调控通路和在应对白僵菌侵染过程中的作用机制。有关研究结果不仅有助于阐明家蚕抵御白僵菌侵染的分子机理，也将为农林害虫的生物、遗传防治提供参考依据。

以筛选、获得的家蚕贮存蛋白基因为研究对象，通过克隆、表达贮存蛋白，研究其表达调控模式和与之互作的基因，及其与白僵菌在家蚕体内、体外的相互作用关系，分析其调控通路和在应对白僵菌侵染过程中的作用机制。完成预定的研究目标，克隆并研究了家蚕贮存蛋白BmSP2、Bm30K-19G1和BmSP1等基因在抵御球孢白僵菌感染过程中的作用。筛选了家蚕感染白僵菌后的差异表达蛋白，并遴选出对白僵菌起抑制作用的药物盐酸特比萘芬，研究了其对中肠微生物菌群的影响,有关研究结果不仅有助于阐明家蚕抵御白僵菌侵染的分子机理，也将为农林害虫的生物、遗传防治提供参考依据。