水环境中酚类内分泌干扰物分子印迹磁性材料选择性识别及吸附效能研究

With the development of industry, endocrine disrupting chemicals (EDCs) were released into the environment, which brings about a plenty of harm to human beings and other living creatures. EDCs go into water body via atmosphere sedimentation, surface runoff, soil eluviation, direct discharge, etc., water body becomes one of the main places where EDCs gather around. Therefore interest in the rapid, precise separation and determination of EDCs in water environment has increased in recent years. It is in urgent need of development of new, selective separation and accumulation material, and sensitive detection technique for removing and monitoring of EDCs in water environment. In this project, molecular imprinting polymers (MIP) will be synthesized, using several phenol EDCs as template moleculars. The novel molecular imprinting magnetic stir bar sorptive extraction will be developed, using MIP as the coating of stir bar, which will achieve desirable selectivity and efficiency for phenol EDCs separation. Combining the molecular imprinting magnetic stir bar sorptive extraction with chemiluminescence detection technique, we aim to develop some of novel rapid, simple, sensitive and selective methods for phenol EDCs analysis. The stir bar coated with MIP can eliminate the interference of complex matrix, which can achieve selective adsorption and reutilization for phenol EDCs. It possesses higher extraction capacity, self-stirring and magnetic separating characteristics, which can avoid competitive adsorption from additional stirrer and toxicity from magnetic nanophase materials. Meanwhile, the sensitized chemiluminescence system can futher improve its sensitivity. This project will help to exploit new ways and methods for water pollution improvement and water environmental recovery.

随着工业生产的发展，内分泌干扰物不断释放到环境中，并通过大气沉降、地表径流、土壤淋溶和直接排放等方式进入水体，给人类和生物带来许多危害。准确、快速地对这些污染物进行分离和分析是环境保护与治理的重要内容之一。因此迫切需要开发新型、高效分离富集材料与高灵敏度分析方法。本项目以双酚A、二氯酚等酚类内分泌干扰物为研究对象，合成分子印迹聚合物，将其涂布于自制内封磁芯的玻璃载体上，制成分子印迹吸附萃取磁性搅拌棒，实现污染物的有效富集和分离，并结合化学发光分析，开发快速、简便、灵敏、选择性的检测新方法。本研究克服了复杂基质的内源性干扰，对目标分子可实现选择性吸附和回收再利用；在萃取时自身完成搅拌和磁分离，简化前处理操作，避免外加搅拌子的竞争吸附和引入磁性纳米材料的毒性污染问题，同时提高了检测的灵敏度。该研究将有助于水污染治理的新途径和新方法，对我国水污染控制和水环境恢复水平提升具有重要意义。

随着工业生产的发展，内分泌干扰物不断释放到环境中，并通过大气沉降、地表径流、土壤淋溶和直接排放等方式进入水体，给人类和生物带来许多危害。准确、快速地对这些污染物进行分离和分析是环境保护与治理的重要内容之一。分子印迹聚合物作为一种新型的分子识别材料，对目标物质具有卓越的分子特异性识别性能。本项目结合分子印迹和磁性分离技术的优势，以双酚A、雌二醇等典型内分泌干扰物为研究对象，制备对目标分子具有预定识别性的分子印迹聚合物，将其负载于磁性载体上，制成分子印迹磁性吸附材料，阐明其分子识别机制，实现对污染物的有效富集和分离。系统考察了制备过程中目标分子、功能单体、交联剂等合成原料的最佳投加量和配比，对影响分子印迹磁性材料吸附和解吸效果的参数进行优化，制备高特异性的选择性吸附材料。优化磁性负载材料，进一步提高分子印迹磁性材料的吸附能力和效率。将分子印迹分离技术与化学发光和高效液相色谱检测技术相结合，开发灵敏、选择性的内分泌干扰物检测新方法。本研究克服了复杂基质的内源性干扰，对目标分子可实现选择性吸附和回收再利用；在萃取时自身完成搅拌和磁分离，简化前处理操作，同时提高了检测的灵敏度。该研究将有助于水污染治理的新途径和新方法，对我国水污染控制和水环境恢复水平提升具有重要意义。