基于共轭聚合物能量转移体系的肺腺癌分子诊断研究

Lung cancer has become a major malignant tumor for its highest mortality rate. Lung adenocarcinoma is the most important histological type of lung cancer. The early diagnosis and choice of treatment are key to improve the survival rate of patients with lung adenocarcinoma. Tumor molecular diagnosis has advantages in early diagnosis, prognosis and targeted therapy. As a novel fluorescent probes, cationic conjugated polymers provide a new method for molecular diagnosis of tumor. Conjugated polymers can form efficient combination of negatively charged DNA molecules by non covalent bonds. In addition, conjugated polymers provides efficient resonance energy transfer with small molecule fluorescent dye fluorescein for light trapping and amplification effect. In this study, we will investigate of relationship between molecular diagnosis in lung adenocarcinoma and targeted therapy using the cationic conjugated polymer based on energy transfer technology (CCP-FRET). The gene mutation and methylation will be detected to screen and identify the molecular diagnosis of lung adenocarcinoma specific markers. DNA methylation analysis for lung adenocarcinoma early diagnosis will be established to study its value in early diagnosis of lung adenocarcinoma, and the correlation between lung adenocarcinoma-associated gene status and tumorigenesis, treatment and prognosis. The high sensitivity detection method, screening key gene molecular markers of lung adenocarcinoma, provides an important basis for the molecular diagnosis and treatment of lung adenocarcinoma.

肺癌已成为致死率首位的恶性肿瘤，肺腺癌是肺癌中最主要的组织学类型，早期诊断和治疗方式的选择是提高肺腺癌患者生存率的关键。肿瘤的分子诊断在早期诊断、判断预后及靶向治疗方面具有优势。共轭聚合物作为一种新型的荧光探针分子，能够以非共价键形式高效结合带负电的DNA分子，具有光捕获和放大效应，与荧光素等小分子荧光染料发生有效地能量共振转移，为肿瘤分子诊断提供了新的策略。本研究利用阳离子共轭聚合物为基础的能量转移技术（CCP-FRET），开展肺腺癌分子诊断和靶向治疗的相关性研究，检测肺腺癌相关基因突变和甲基化修饰位点，筛选和确定肺腺癌特异性的分子诊断标志物，建立用于肺腺癌早期诊断的DNA甲基化分析方法，研究其在肺腺癌早期诊断中的价值，分析肺腺癌相关基因状态与肿瘤发生、治疗以及预后的相关性。建立高灵敏感的检测方法，筛选肺腺癌关键基因的分子诊断标志物，为肺腺癌分子诊断和治疗提供重要基础。

肺癌已成为致死率首位的恶性肿瘤，肺腺癌是肺癌中最主要的组织学类型，早期诊断和治疗方式的选择是提高肺腺癌患者生存率的关键。肿瘤的分子诊断在早期诊断、判断预后及靶向治疗方面具有优势。共轭聚合物作为一种新型的荧光探针分子，能够以非共价键形式高效结合带负电的DNA分子，具有光捕获和放大效应，与荧光素等小分子荧光染料发生有效地能量共振转移，为肿瘤分子诊断提供了新的策略。本研究利用阳离子共轭聚合物为基础的能量转移技术（CCP-FRET），开展肺腺癌分子诊断和靶向治疗的相关性研究，检测了肺腺癌相关基因突变和甲基化修饰位点，建立了用于肺腺癌的DNA甲基化分析方法，分析了肺腺癌相关基因状态与肿瘤发生、治疗以及预后的相关性。建立高灵敏感的检测方法，筛选肺癌关键基因的分子诊断标志物，为肺腺癌分子诊断和治疗提供重要基础。