食品包装纳米阻隔活性双重内涂层膜的复合、阻隔与抗菌机制

In order to improve the shelf-life and safety of food, montmorillonite nanolayer barrier active double inner coating membranes are prepared by coating technique. The synergistic effect of barrier and antimicrobial activity is realized through the intercoupling between barrier and antimicrobial coating membrane. The system of soybean protein isolate intercalated MMT/EVOH nanolayer compound barrier coating membrane is established by structure design and molecule modification. The intercalation mechanism of soybean protein isolate to MMT and composite mechanism of modified MMT to EVOH are investigated. The dispersion morphology and orientation for the nanolayer of MMT in EVOH matrix are controlled by shearing force produced during coating process. The migration behavior and barrier mechanism of aimed substance are explored. The system of model antimicrobial agent Nisin loaded poly（lactic acid)/ chitosan active membrane is established on barrier coating membrane by structure design and function recombination. The common bacteria of food such as St. aurens，E. coli and B. subtilis are chosen as tested bacteria. The synergistic effect of chitosan and model antimicrobial agent Nisin, and the mechanism of controlled-release are focused on. This research result will provide novel method and theoretical foundation for the design and fabrication of new safety barrier active food packaging material. soybean protein isolate, poly（lactic acid) and chitosan are originated from agricultural byproduct, thus this project is benefit to the comprehensive utilization and high value-added of agricultural byproduct.

以延长食品货架期、保证食品安全为目的，采用涂覆技术，制备食品包装蒙脱土纳米片阻隔活性双重内涂层膜。通过阻隔层与活性层的耦合，实现阻隔和抗菌性能的协同。通过结构设计与分子修饰，建立SPI插层MMT/EVOH纳米复合阻隔体系，研究SPI插层MMT及其与EVOH复合机理；通过涂覆过程中的剪切力，控制MMT纳米片层在EVOH基体中分散形态与取向；揭示目标物质在阻隔层中的迁移行为及阻隔机制。通过结构设计与性能重组，建立模型抗菌剂Nisin负载聚L-乳酸/壳聚糖活性层体系；选择食品中常见的St. aurens，E. coli和B. subtilis作为受试菌，研究壳聚糖、模型抗菌剂Nisin协同抗菌以及控释机制。研究成果将为新型安全阻隔活性食品包装材料的设计与制备提供新思路和理论基础。大豆蛋分离白、聚L-乳酸、壳聚糖源于农副产品，有利于农副产品的综合利用和附加值的提高。

采用涂膜技术制备蒙脱土纳米片层阻隔活性双重内涂层膜，实现阻隔和抗菌性能的协同；建立大豆蛋白（SPI）插层MMT/EVOH纳米复合阻隔体系和nisin负载聚L-乳酸/壳聚糖活性层体系；研究壳聚糖、模型抗菌剂nisin协同抗菌以及控释机制。在此基础上，进一步拓展了高分子基体、无机纳米粒子及抗菌剂类型。项目执行过程中，形成了系列食品活性包装材料。.涂膜法制备nisin-壳聚糖/聚L乳酸和乳酸钠-壳聚糖/聚L乳酸以及nisin-壳聚糖/聚乙烯醇（和乳酸钠-壳聚糖/聚乙烯醇/蒙脱土阻隔抗菌薄膜，评价其光学、力学、阻隔和抗菌性能，揭示膜中nisin 和乳酸钠的扩散行为，建立阻隔与抗菌模型，获得相关动力学和热力学数据。利用磷酸化大豆蛋白插层MMT（MMT-PSPI）与EVOH复合制备EVOH-MMT-PSPI纳米阻隔薄膜。提出并证实了EVOH/MMT–PSPI的结构，揭示MMT-PSPI的含量对膜的透光、力学、热学和阻隔性能的相关性；设计空间填充嵌入技术制备MMT负载壳聚糖-聚氧化乙烯纳米纤维（MMT@CPF）增强EVOH纳米阻隔膜(EVOH-MMT@CPF)。提出MMT@CPF增强EVOH机制，评价膜的力学、热学和阻隔性能。高压平行电场中实现功能化石墨烯在EVOH的自组装，制备出甲氧基苯功能化石墨烯/ EVOH LbL阻隔膜和抗菌甲基丙烯酰氧乙基三甲基氯化铵功能化石墨烯/ EVOH LbL阻隔膜，评价膜的力学、阻隔与抗菌性能及生理毒性。.为深入研究隔活性双重内涂层的扩散与抗菌机制，静电纺丝制备出系列具有活性包装特性的功能纤维膜，探索了功能物质（原花青素、姜黄素、nisin，盐酸四环素）在高分子基体中的抗菌、抗氧化和控释行为以及纤维对溶液中的重金属离子Co（II）的吸附行为。围绕抗氧化和抗菌性能，获得几种功能淀粉，包括高吸附多孔糊化玉米淀粉、多孔壳聚糖改性淀粉和锌(II) 功能化L-苯丙氨酸原位接枝改性淀粉（Zn(II)@L-Phe-g-St）。研究多孔淀粉吸附行为及Zn(II)@L-Phe-g-St的抗菌性能和的毒理性。.研究成果在食品活性包装材料方面具有广阔的应用前景，并为新型食品活性包装材料的设计提供理论基础和技术支持。