基于可破解有机硅纳米胶囊的食源性致病菌的压电、电化学、荧光多重信号放大检测

Foodborne pathogens have brought great threat to human health, so it is urgent to develop rapid, accurate and sensitive methods for the detection of pathogens. This project is proposed to prepare novel fluorescent nanocapsules by filling carbon dots, Au nanoclusters, CdS quantum dots, etc. in breakable organosilica nanocapsules, ensuring that a large number of quantum dots are encapsulated in each nanocapsule. To develop nanocapsule labels that can specifically bind to target pathogens, the as-prepared nanocapsules are subjected to surface amination, and then conjugated with biological recognition molecules such as antibodies and aptamers. The target pathogens are captured by biological recognition molecules modified piezoelectric quartz crystal electrode, and then labeled with fluorescent nanocapsules, to construct sandwich-type biosensors. Piezoelectric and electrochemical detection of pathogens are carried out by monitoring various modification steps with piezoelectric quartz crystal microbalance and electrochemical impedance spectroscopy. Fluorescent nanoparticles are released after destructing the organosilica nanocapsules, and then detection of pathogens is carried out. By using the novel fluorescent nanocapsules as labels, piezoelectric, electrochemical and fluorescent multiplex signals can be amplified, so the sensitivities and accuracy for the detection of pathogens can be improved. This project is expected to develop a new method for the detection of foodborne pathogens, and thus provides supplementary methods and reliable scientific basis for public safety, food hygiene, medical diagnosis, and environmental testing.

食源性致病菌对人类健康构成了巨大威胁，因此亟需建立致病菌的快速、准确、灵敏的检测方法。本项目拟将碳点、金纳米团簇、CdS量子点等封装在可破解的有机硅纳米胶囊内，制备新型荧光纳米胶囊，确保每个纳米胶囊内均能封装大量的荧光纳米颗粒。对荧光纳米胶囊进行表面氨基化，修饰抗体、核酸适体等生物识别分子，发展能特异性结合目标致病菌的胶囊标记物。利用生物识别分子修饰的压电石英晶体电极捕获目标致病菌，再标记荧光纳米胶囊，构建三明治结构生物传感器。利用石英晶体微天平、电化学交流阻抗技术对传感器的构建过程进行表征，实现致病菌的压电、电化学检测。将有机硅纳米胶囊破解，释放荧光纳米颗粒，实现致病菌的荧光检测。以新型荧光纳米胶囊为标记物，可实现压电、电化学、荧光多重信号的放大，提高致病菌检测的灵敏度和准确性。本项目有望发展食源性致病菌的检测新方法，为公共安全、食品卫生、医学诊断、环境检测提供可靠的补充方法和科学依据。

食源性致病菌对人类健康构成了巨大威胁，因此亟需建立致病菌的快速、准确、灵敏的检测方法。本项目将碳点、金纳米团簇、CdS量子点等封装在可破解的有机硅纳米胶囊内，制备了新型荧光纳米胶囊，确保了每个纳米胶囊内均能封装大量的荧光纳米颗粒。对荧光纳米胶囊进行表面氨基化，修饰抗体，发展了能特异性结合目标致病菌的胶囊标记物。利用抗体修饰的压电石英晶体电极捕获目标致病菌，再标记荧光免疫纳米胶囊，构建了三明治结构生物传感器。利用压电石英晶体微天平、电化学交流阻抗技术对传感器的构建过程进行了表征，实现了致病菌的压电、电化学检测；将有机硅纳米胶囊破解，释放荧光纳米颗粒，实现了致病菌的荧光检测。由于每个免疫纳米胶囊内均封装了大量的荧光纳米颗粒，以新型荧光纳米胶囊为标记物，极大地提高了致病菌检测的灵敏度。所构建的生物传感器能输出压电、电化学、荧光多重信号，提高了致病菌检测的准确性。在该课题的资助下，项目负责人已以通讯作者或第一作者在ACS Applied Materials & Interfaces、Talanta和Analyst等期刊发表论文6篇。